gamma-catenin Antibody (H-1) recommended for detection of γ-catenin of human origin by WB, IP, IF, IHC(P) and ELISA

γ-catenin Antibody (H-1): sc-8415

γ-catenin Antibody (H-1) is rated 5.0 out of 5 by 5.
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Datasheets

    • γ-catenin Antibody (H-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 30-109 mapping near the N-terminus of γ-catenin of human origin
    • recommended for detection of γ-catenin of human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can γ-catenin (H-1): sc-8415 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, γ-catenin (H-1): sc-8415 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Publishable IP/WB results in human HN5 samples Publishable IP/WB results in human HN5 samples. -SCBT Publication Review
Date published: 2015-04-24
Rated 5 out of 5 by from Successful western blot detection of Successful western blot detection of -catenin expression in HeLa and A-431 whole cell lysates. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by from Worked well in immunofluorescence staining Worked well in immunofluorescence staining of methanol-fixed HeLa cells, showing membrane and cell junction localization. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from IHC(P) and IF data published in human pancreas IHC(P) and IF data published in human pancreas samples. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Positive immunoperoxidase staining of formalin Positive immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma showed membrane localization -SCBT QC
Date published: 2013-07-11
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