G_{α t1} Antibody (F-12): sc-518142

Gα t1 Antibody (F-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Gα t1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Gα t1 antibody (F-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gα t1 is a member of the Gi class of heterotrimeric G proteins, which play a crucial role in cellular signaling by transmitting signals from activated receptors on the cell surface to intracellular effectors. Specifically, Gα t1 is involved in the visual signaling pathway, where Gα t1 interacts with photorhodopsin to facilitate the exchange of guanine nucleotides, a process essential for phototransduction in rod cells. This interaction enables conversion of light signals into electrical signals, allowing for vision in low-light conditions. Proper functioning of Gα t1 and associated pathways maintains visual acuity and overall eye health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

G_{α t1} Antibody (F-12) References:

1. A nonsense mutation in Gnat1, encoding the alpha subunit of rod transducin, in spontaneous mouse models of retinal dysfunction.  |  Miyamoto, M., et al. 2010. Exp Eye Res. 90: 63-9. PMID: 19766629
2. GNAT1 associated with autosomal recessive congenital stationary night blindness.  |  Naeem, MA., et al. 2012. Invest Ophthalmol Vis Sci. 53: 1353-61. PMID: 22190596
3. A novel homozygous truncating GNAT1 mutation implicated in retinal degeneration.  |  Carrigan, M., et al. 2016. Br J Ophthalmol. 100: 495-500. PMID: 26472407
4. Nucleotide sequence for a cDNA encoding the alpha subunit of retinal transducin (GNAT1) isolated from the human eye.  |  Van Dop, C., et al. 1989. Nucleic Acids Res. 17: 4887. PMID: 2748346
5. Identification of a Novel Homozygous Nonsense Mutation Confirms the Implication of GNAT1 in Rod-Cone Dystrophy.  |  Méjécase, C., et al. 2016. PLoS One. 11: e0168271. PMID: 27977773
6. A Novel Heterozygous Missense Mutation in GNAT1 Leads to Autosomal Dominant Riggs Type of Congenital Stationary Night Blindness.  |  Zeitz, C., et al. 2018. Biomed Res Int. 2018: 7694801. PMID: 29850563
7. Coexistence of GNAT1 and ABCA4 variants associated with Nougaret-type congenital stationary night blindness and childhood-onset cone-rod dystrophy.  |  Hayashi, T., et al. 2020. Doc Ophthalmol. 140: 147-157. PMID: 31583501
8. Sleep Deprivation Does not Change the Flash Electroretinogram in Wild-type and Opn4-/-Gnat1-/- Mice.  |  Schoonderwoerd, RA., et al. 2022. J Biol Rhythms. 37: 216-221. PMID: 35132885
9. Regional mapping of a human rod alpha-transducin (GNAT1) gene to chromosome 3p22.  |  Ngo, JT., et al. 1993. Genomics. 18: 724-5. PMID: 8307584
10. A highly polymorphic RFLP marker in the canine transducin alpha-1 subunit gene (GNAT1).  |  Ray, K., et al. 1996. Anim Genet. 27: 372-3. PMID: 8930085

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α t1} Antibody (F-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α t1} Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α t1} Antibody (F-12) Specifications

Alternate Product Name: Gα t1

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 114-131

Epitope Target: G_{α t1}

Epitope Species: mouse

Product Reactivity/Detection: G_{α t1}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for G_{α t1} Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred G_{α t1} antibody is G_{α t1} Antibody (C-9): sc-518139.