G_{α s/olf} Antibody (A-5): sc-55545

Gα s/olf Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody engineered to recognize the Gα s and Gα olf subunits of heterotrimeric G proteins, also known as the G-protein subunit from the GNAS locus or GNAL Olfactory antibody. Gα s/olf monoclonal antibody (A-5) targets an epitope comprising amino acids 82-381 at the C-terminus of the human Gα s/olf protein, ensuring robust and precise binding. Gα s and Gα olf are integral to cellular signal transduction, facilitating the relay of information from diverse cell surface receptors to intracellular effectors such as adenylyl cyclase, thereby modulating critical physiological processes like hormone secretion and olfactory perception. By translating extracellular signals into specific cellular responses, these G protein subunits play a pivotal role in maintaining various physiological functions, including metabolism, sensory detection, and neuronal communication. Gα s/olf (A-5) antibody demonstrates broad species reactivity, effectively recognizing Gα s1-4 and Gα olf proteins from mouse, rat, and human origins, making a versatile tool for cross-species studies. Gα s/olf (A-5) monoclonal antibody is compatible with a range of laboratory techniques, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), enabling comprehensive analysis of G protein-mediated pathways. Additionally, anti-Gα s/olf antibody (A-5) is available in both non-conjugated forms and multiple conjugated variants such as horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® labels, providing flexibility to accommodate different experimental requirements. Gα s/olf (A-5) monoclonal antibody enables researchers to gain deeper insights into the mechanisms of G protein signaling and its implications in health and disease, thereby facilitating advancements in biomedical research.

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G_{α s/olf} Antibody (A-5) References:

1. Different beta-subunits determine G-protein interaction with transmembrane receptors.  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
2. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
3. G protein beta gamma subunits synthesized in Sf9 cells. Functional characterization and the significance of prenylation of gamma.  |  Iñiguez-Lluhi, JA., et al. 1992. J Biol Chem. 267: 23409-17. PMID: 1429682
4. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
5. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
6. Investigation of the G protein subunit Galphaolf gene (GNAL) in attention deficit/hyperactivity disorder.  |  Laurin, N., et al. 2008. J Psychiatr Res. 42: 117-24. PMID: 17166517
7. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
8. Golf: an olfactory neuron specific-G protein involved in odorant signal transduction.  |  Jones, DT. and Reed, RR. 1989. Science. 244: 790-5. PMID: 2499043
9. G protein subunit phosphorylation as a regulatory mechanism in heterotrimeric G protein signaling in mammals, yeast, and plants.  |  Chakravorty, D. and Assmann, SM. 2018. Biochem J. 475: 3331-3357. PMID: 30413679
10. G protein subunit alpha i2's pivotal role in angiogenesis.  |  Bai, CW., et al. 2024. Theranostics. 14: 2190-2209. PMID: 38505600
11. Selectivity in signal transduction determined by gamma subunits of heterotrimeric G proteins.  |  Kleuss, C., et al. 1993. Science. 259: 832-4. PMID: 8094261
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α s/olf} Antibody (A-5) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α s/olf} Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α s/olf} Antibody (A-5) Specifications

Alternate Product Name: G-protein subunit GNAS locus / GNAL Olfactory

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 82-381

Epitope Target: Gα s/olf

Epitope Species: human

Product Reactivity/Detection: G_{α s1-4} and G_{α olf}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for G_{α s/olf} Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred G_{α s} antibody is G_{α s} Antibody (12): sc-135914.