G_{α o} Antibody (E-1): sc-393874

Gα o Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gα o in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gα o (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gα o protein plays a crucial role in cellular signaling as a member of the heterotrimeric G protein family, which transmits signals from cell surface receptors to intracellular effectors. Specifically, Gα o is involved in the modulation of ion channels and the regulation of neurotransmitter release, making Gα o essential for processes such as synaptic transmission and muscle contraction. Gα o′s ability to bind and hydrolyze guanosine triphosphate (GTP) allows Gα o to act as a molecular switch, toggling between active and inactive states in response to receptor activation. This dynamic regulation is vital for maintaining cellular homeostasis and responding to external stimuli, highlighting Gα o′s importance in various physiological processes.

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G_{α o} Antibody (E-1) References:

1. N-Myristoylation and betagamma play roles beyond anchorage in the palmitoylation of the G protein alpha(o) subunit.  |  Wang, Y., et al. 1999. J Biol Chem. 274: 37435-42. PMID: 10601316
2. Translocation of activated heterotrimeric G protein Galpha(o) to ganglioside-enriched detergent-resistant membrane rafts in developing cerebellum.  |  Yuyama, K., et al. 2007. J Biol Chem. 282: 26392-400. PMID: 17623667
3. Regulator of G protein signaling protein suppression of Galphao protein-mediated alpha2A adrenergic receptor inhibition of mouse hippocampal CA3 epileptiform activity.  |  Goldenstein, BL., et al. 2009. Mol Pharmacol. 75: 1222-30. PMID: 19225179
4. The G protein alpha o subunit alters morphology, growth kinetics, and phospholipid metabolism of somatic cells.  |  Bloch, DB., et al. 1989. Mol Cell Biol. 9: 5434-9. PMID: 2511433
5. Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.  |  Solis, GP., et al. 2017. Cell. 170: 939-955.e24. PMID: 28803726
6. Golgi-Resident Gαo Promotes Protrusive Membrane Dynamics.  |  Solis, GP., et al. 2017. Cell. 170: 1258. PMID: 28886387
7. Inhibitory signaling in mammalian olfactory transduction potentially mediated by Gαo.  |  Corey, EA., et al. 2021. Mol Cell Neurosci. 110: 103585. PMID: 33358996
8. Restoration of the GTPase activity and cellular interactions of Gαo mutants by Zn2+ in GNAO1 encephalopathy models.  |  Larasati, YA., et al. 2022. Sci Adv. 8: eabn9350. PMID: 36206333
9. Palmitoylation of RGS20 affects Gαo-mediated signaling independent of its GAP activity.  |  Zhang, Q. and Sjögren, B. 2023. Cell Signal. 107: 110682. PMID: 37075876
10. GNAO1 Mutations Affecting the N-Terminal α-Helix of Gαo Lead to Parkinsonism.  |  Solis, GP., et al. 2024. Mov Disord. 39: 601-606. PMID: 38358016

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{α o} Antibody (E-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{α o} Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{α o} Antibody (E-1) Specifications

Alternate Product Name: G protein subunit GNAO

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 40-291

Epitope Target: G_{α o}

Epitope Species: human

Product Reactivity/Detection: G_{α o}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for G_{α o} Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred G_{α o} antibody is G_{α o} Antibody (A2): sc-13532.