G_{α 12} Antibody (B-5): sc-515610

Gα 12 Antibody (B-5) is a mouse monoclonal IgG2a antibody that detects Gα 12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gα 12 is a member of the heterotrimeric G protein family, which plays a crucial role in transmitting signals from cell surface receptors to various intracellular effectors, thereby influencing numerous physiological processes. Gα 12 monoclonal antibody (B-5) helps researchers study pathways that control cell proliferation, migration, and differentiation, making Gα 12 essential for normal cellular function and development. Gα 12 is primarily located at the plasma membrane, where Gα 12 interacts with various receptors and downstream signaling molecules, facilitating the transduction of extracellular signals into cellular responses. This localization allows Gα 12 to effectively relay information from a diverse array of stimuli, including hormones and neurotransmitters, to intracellular effectors, such as Rho guanine nucleotide exchange factors, which subsequently activate Rho GTPases. The ability of Gα 12 to modulate these pathways underscores Gα 12′s importance in maintaining cellular homeostasis and potential implications in various diseases, including cancer and cardiovascular disorders. Anti-Gα 12 antibody (B-5) is an invaluable tool for researchers aiming to explore the intricate signaling mechanisms mediated by Gα 12 and its role in health and disease.

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G_{α 12} Antibody (B-5) References:

1. Galpha12 and Galpha13 as key regulatory mediator in signal transduction.  |  Kurose, H. 2003. Life Sci. 74: 155-61. PMID: 14607242
2. The gep oncogenes, Galpha(12) and Galpha(13), upregulate the transforming growth factor-beta1 gene.  |  Lee, SJ., et al. 2009. Oncogene. 28: 1230-40. PMID: 19151758
3. G protein α12 (Gα12) is a negative regulator of kidney injury molecule-1-mediated efferocytosis.  |  Ismail, OZ., et al. 2016. Am J Physiol Renal Physiol. 310: F607-F620. PMID: 26697979
4. Gα12 regulates osteoclastogenesis by modulating NFATc1 expression.  |  Song, MK., et al. 2018. J Cell Mol Med. 22: 849-860. PMID: 29077264
5. Gα12 ablation exacerbates liver steatosis and obesity by suppressing USP22/SIRT1-regulated mitochondrial respiration.  |  Kim, TH., et al. 2018. J Clin Invest. 128: 5587-5602. PMID: 30300140
6. Receptor selectivity between the G proteins Gα12 and Gα13 is defined by a single leucine-to-isoleucine variation.  |  Mackenzie, AE., et al. 2019. FASEB J. 33: 5005-5017. PMID: 30601679
7. Downregulation of Brain Gα12 Attenuates Angiotensin II-Dependent Hypertension.  |  Gao, J., et al. 2020. Am J Hypertens. 33: 198-204. PMID: 31677381
8. The emerging roles of Gα12/13 proteins on the hallmarks of cancer in solid tumors.  |  Rasheed, SAK., et al. 2022. Oncogene. 41: 147-158. PMID: 34689178
9. Insights into lysophosphatidylserine recognition and Gα12/13-coupling specificity of P2Y10.  |  Yin, H., et al. 2024. Cell Chem Biol. 31: 1899-1908.e5. PMID: 39265572
10. Synaptic Gα12/13 signaling establishes hippocampal PV inhibitory circuits.  |  Garbett, K., et al. 2024. Proc Natl Acad Sci U S A. 121: e2407828121. PMID: 39693341

The preferred G_{α 12} antibody is G_{α 12} Antibody (E-12): sc-515445.