GAL4-TA Antibody (A-2): sc-46680
- GAL4-TA Antibody (A-2) is a mouse monoclonal IgG1 κ GAL4-TA antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 768-881 mapping within the acidic activator domain of GAL4-TA
- GAL4-TA Antibody (A-2) is recommended for detection of GAL4-TA by WB, IP, IF and ELISA
- Anti-GAL4-TA Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GAL4-TA Antibody (A-2) for WB applications. These reagents are now offered in bundles with GAL4-TA Antibody (A-2) (see ordering information below).
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GAL4-TA Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects GAL4-TA protein of Saccharomyces cerevisiae by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GAL4-TA (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAL4-TA functions as a crucial transcriptional activator, regulating gene expression in response to galactose. The transcriptional activation domain, spanning C-terminal amino acids 768-881, activates GAL genes including GAL1, GAL2, GAL7, GAL10, and MEL1, which is essential for galactose metabolism. The N-terminal 147 amino acid residues of GAL4-TA exhibit specific and strong DNA binding properties, making this fragment valuable for studying transcriptional activation functions of various heterologous proteins. GAL4-TA′s unique properties have enabled development of "two-hybrid" systems in yeast for identifying specific protein-protein interactions, highlighting GAL4-TA′s significance in molecular biology research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GAL4-TA Antibody (A-2) References:
- Mitochondrial dysfunction enhances Gal4-dependent transcription. | Jelicić, B., et al. 2005. FEMS Microbiol Lett. 253: 207-13. PMID: 16239078
- The expanding reach of the GAL4/UAS system into the behavioral neurobiology of Drosophila. | Jones, WD. 2009. BMB Rep. 42: 705-12. PMID: 19944010
- cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans. | Wang, H., et al. 2017. Nat Methods. 14: 145-148. PMID: 27992408
- Drosophila enhancer-Gal4 lines show ectopic expression during development. | Casas-Tintó, S., et al. 2017. R Soc Open Sci. 4: 170039. PMID: 28405401
- Genetic Reagents for Making Split-GAL4 Lines in Drosophila. | Dionne, H., et al. 2018. Genetics. 209: 31-35. PMID: 29535151
- GAL4 Drivers Specific for Type Ib and Type Is Motor Neurons in Drosophila. | Pérez-Moreno, JJ. and O'Kane, CJ. 2019. G3 (Bethesda). 9: 453-462. PMID: 30530644
- Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells. | Nagasaki, SC., et al. 2023. Cell Struct Funct. 48: 31-47. PMID: 36529516
- A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila. | Qian, Y., et al. 2023. ACS Synth Biol. 12: 664-671. PMID: 36891673
- The intestinal stem cell/enteroblast-GAL4 driver, escargot-GAL4, also manipulates gene expression in the juvenile hormone-synthesizing organ of Drosophila melanogaster. | Kurogi, Y., et al. 2024. Sci Rep. 14: 9631. PMID: 38671036
- The GAL4 system as a tool for unravelling the mysteries of the Drosophila nervous system. | Brand, AH. and Dormand, EL. 1995. Curr Opin Neurobiol. 5: 572-8. PMID: 8580708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAL4-TA Antibody (A-2) | sc-46680 | 200 µg/ml | $322.00 | |||
GAL4-TA Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-526724 | 200 µg Ab; 10 µg BP | $361.00 | |||
GAL4-TA Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-532097 | 200 µg Ab; 20 µg BP | $361.00 | |||
GAL4-TA Antibody (A-2) AC | sc-46680 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GAL4-TA Antibody (A-2) HRP | sc-46680 HRP | 200 µg/ml | $322.00 | |||
GAL4-TA Antibody (A-2) FITC | sc-46680 FITC | 200 µg/ml | $336.00 | |||
GAL4-TA Antibody (A-2) PE | sc-46680 PE | 200 µg/ml | $349.00 | |||
GAL4-TA Antibody (A-2) Alexa Fluor® 488 | sc-46680 AF488 | 200 µg/ml | $364.00 | |||
GAL4-TA Antibody (A-2) Alexa Fluor® 546 | sc-46680 AF546 | 200 µg/ml | $364.00 | |||
GAL4-TA Antibody (A-2) Alexa Fluor® 594 | sc-46680 AF594 | 200 µg/ml | $364.00 | |||
GAL4-TA Antibody (A-2) Alexa Fluor® 647 | sc-46680 AF647 | 200 µg/ml | $364.00 | |||
GAL4-TA Antibody (A-2) Alexa Fluor® 680 | sc-46680 AF680 | 200 µg/ml | $364.00 | |||
GAL4-TA Antibody (A-2) Alexa Fluor® 790 | sc-46680 AF790 | 200 µg/ml | $364.00 |