GAL4-TA Antibody (C-10): sc-1663

GAL4-TA Antibody (C-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects GAL4-TA through western blotting (WB) and immunoprecipitation (IP). anti-GAL4-TA antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAL4, derived from Saccharomyces cerevisiae, is a well-studied transcriptional activator known for its critical role in gene expression regulation. One key aspect of GAL4 is forming specific protein-protein interactions, particularly with other transcription factors and co-activators, which is essential for transcriptional activation. These interactions facilitate recruitment of transcriptional machinery to target genes, enhancing gene expression. GAL4′s versatility in forming fusions with various activating domains has led to widespread use in developing innovative experimental systems, such as the "two-hybrid" system, which allows researchers to explore and identify specific protein interactions in yeast, plants, insects, and mammalian cells. GAL4-TA (C-10) antibody serves as an invaluable tool for researchers investigating transcriptional regulation and protein interactions.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GAL4-TA Antibody (C-10) References:

1. GAL4 fusion vectors for expression in yeast or mammalian cells.  |  Sadowski, I., et al. 1992. Gene. 118: 137-41. PMID: 1511877
2. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.  |  Chien, CT., et al. 1991. Proc Natl Acad Sci U S A. 88: 9578-82. PMID: 1946372
3. Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.  |  Song, D., et al. 1991. Genes Dev. 5: 741-50. PMID: 2026326
4. Activators and targets.  |  Ptashne, M. and Gann, AA. 1990. Nature. 346: 329-31. PMID: 2142753
5. A novel genetic system to detect protein-protein interactions.  |  Fields, S. and Song, O. 1989. Nature. 340: 245-6. PMID: 2547163
6. Gal4 Driver Transgenic Zebrafish: Powerful Tools to Study Developmental Biology, Organogenesis, and Neuroscience.  |  Kawakami, K., et al. 2016. Adv Genet. 95: 65-87. PMID: 27503354
7. A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.  |  Johnston, M. 1987. Microbiol Rev. 51: 458-76. PMID: 2830478
8. Deletion analysis of GAL4 defines two transcriptional activating segments.  |  Ma, J. and Ptashne, M. 1987. Cell. 48: 847-53. PMID: 3028647
9. Differential efficacy of genetically swapping GAL4.  |  Chen, YJ., et al. 2019. J Neurogenet. 33: 52-63. PMID: 30939963
10. split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.  |  Ewen-Campen, B., et al. 2023. Proc Natl Acad Sci U S A. 120: e2304730120. PMID: 37276389
11. Rbp4-Gal4, a germline driver that activates in meiosis, reveals functions for VCP in spermatid development.  |  Butsch, TJ., et al. 2023. Fly (Austin). 17: 2234795. PMID: 37436409
12. Using single-cell RNA sequencing to generate predictive cell-type-specific split-GAL4 reagents throughout development.  |  Chen, YD., et al. 2023. Proc Natl Acad Sci U S A. 120: e2307451120. PMID: 37523539

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAL4-TA Antibody (C-10) has 32 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAL4-TA Antibody (C-10) Specifications

Alternate Product Name: GAL4-TA

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 768-881

Epitope Target: GAL4-TA

Epitope Species:

Product Reactivity/Detection: GAL4-TA

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for GAL4-TA Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent