GAL4 Antibody (D-11): sc-166317

GAL4 Antibody (D-11) is a mouse monoclonal IgG2a kappa light chain antibody designed to detect the GAL4 protein, also known as the GAL4 DNA-binding domain or yeast GAL4 transcription factor. GAL4 monoclonal antibody (D-11) targets an epitope within amino acids 1-147 of the N-terminal DNA binding domain of GAL4, enabling effective detection in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) across species including mouse, rat, and human. GAL4 is a well-characterized transcriptional activator from Saccharomyces cerevisiae that resides in the nucleus, where GAL4 binds to specific upstream activation sequences (UAS) in DNA to regulate the transcription of target genes. The nuclear localization of GAL4 is essential for controlling gene expression, making GAL4 a pivotal tool in molecular biology for studying gene regulation and protein interactions. GAL4′s ability to mediate strong DNA binding through its N-terminal domain, while requiring fusion with activating domains for transcriptional activation, underpins GAL4′s use in techniques such as the yeast two-hybrid system, which identifies protein-protein interactions. Anti-GAL4 antibody (D-11) is an indispensable reagent for experiments involving GAL4-based systems, contributing to advancements in genetic research, functional assays, and the understanding of transcriptional mechanisms. Additionally, GAL4 (D-11) antibody supports a wide range of experimental designs, enhancing the ability to explore complex biological processes and regulatory pathways in various research contexts.

The preferred GAL4 [DBD] antibody is GAL4 [DBD] Antibody (RK5C1): sc-510.