Gads Antibody (UW40): sc-73652

Gads Antibody (UW40) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gads protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Gads antibody (UW40) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gads protein plays a crucial role as an adapter protein, containing both Src homology 2 (SH2) and Src homology 3 (SH3) domains, which are essential for mediating signal transduction pathways. The presence of these domains allows Gads to bind to tyrosine-phosphorylated proteins, such as Shc, effectively linking them to downstream signaling effectors. This interaction is vital for the regulation of cellular responses to growth factors and other stimuli, highlighting the importance of Gads in maintaining proper cellular signaling and function. Furthermore, the SH3 domain′s ability to interact with various proteins underscores its role in the negative regulation of nonreceptor tyrosine kinases, which can prevent uncontrolled cell proliferation and transformation. Gads monoclonal antibody (UW40) enables researchers to gain valuable insights into the intricate signaling networks that govern cellular behavior in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Gads Antibody (UW40) References:

1. The role of Gads in hematopoietic cell signalling.  |  Liu, SK., et al. 2001. Oncogene. 20: 6284-90. PMID: 11607830
2. Phosphorylation of GAP and GAP-associated proteins by transforming and mitogenic tyrosine kinases.  |  Ellis, C., et al. 1990. Nature. 343: 377-81. PMID: 1689011
3. Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex.  |  Morrison, DK., et al. 1990. Mol Cell Biol. 10: 2359-66. PMID: 1691440
4. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.  |  Koch, CA., et al. 1991. Science. 252: 668-74. PMID: 1708916
5. Oncogenes and signal transduction.  |  Cantley, LC., et al. 1991. Cell. 64: 281-302. PMID: 1846320
6. Gads regulates the expansion phase of CD8+ T cell-mediated immunity.  |  Zhang, EY., et al. 2011. J Immunol. 186: 4579-89. PMID: 21411729
7. Signal transduction by receptors with tyrosine kinase activity.  |  Ullrich, A. and Schlessinger, J. 1990. Cell. 61: 203-12. PMID: 2158859
8. GADS is required for TCR-mediated calcium influx and cytokine release, but not cellular adhesion, in human T cells.  |  Bilal, MY., et al. 2015. Cell Signal. 27: 841-50. PMID: 25636200
9. Expression of GADS enhances FLT3-induced mitogenic signaling.  |  Chougule, RA., et al. 2016. Oncotarget. 7: 14112-24. PMID: 26895103
10. Itk Promotes the Integration of TCR and CD28 Costimulation through Its Direct Substrates SLP-76 and Gads.  |  Hallumi, E., et al. 2021. J Immunol. 206: 2322-2337. PMID: 33931484
11. Interaction of Shc with the zeta chain of the T cell receptor upon T cell activation.  |  Ravichandran, KS., et al. 1993. Science. 262: 902-5. PMID: 8235613
12. Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc.  |  Liu, SK. and McGlade, CJ. 1998. Oncogene. 17: 3073-82. PMID: 9872323

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gads Antibody (UW40) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Gads Antibody (UW40) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gads Antibody (UW40) Specifications

Alternate Product Name: Gads

Product Clone Name: UW40

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Gads

Epitope Species: human

Product Reactivity/Detection: Gads

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Gads Antibody (UW40): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin