GAD-67 Antibody (A-4): sc-390383

GAD-67 Antibody (A-4) is a mouse monoclonal IgG3 antibody that detects glutamic acid decarboxylase 67 (GAD-67) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GAD-67 plays a crucial role in the synthesis of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system, which is essential for maintaining the balance between neuronal excitation and inhibition. This balance is vital for normal brain function, as disruptions can lead to various neurological disorders, including epilepsy and anxiety. GAD-67 is predominantly localized in the cytoplasm of neurons, where GAD-67 is responsible for the basal production of GABA, ensuring that adequate levels are maintained for neurotransmission. In contrast, GAD-65, which is activated during periods of increased GABA demand, is found in synaptic vesicle membranes, highlighting the complementary roles of these two isoforms in GABAergic signaling. Anti-GAD-67 antibody (A-4) is an invaluable tool for researchers studying GABA synthesis and its implications in neurobiology, providing insights into the mechanisms underlying various neurological conditions.

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GAD-67 Antibody (A-4) References:

1. Increased expression of glutamate decarboxylase (GAD(67)) in feline lumbar spinal cord after complete thoracic spinal cord transection.  |  Tillakaratne, NJ., et al. 2000. J Neurosci Res. 60: 219-30. PMID: 10740227
2. Decrease in GABA synthesis rate in rat cortex following GABA-transaminase inhibition correlates with the decrease in GAD(67) protein.  |  Mason, GF., et al. 2001. Brain Res. 914: 81-91. PMID: 11578600
3. PDX-1 mediates glucose responsiveness of GAD(67), but not GAD(65), gene transcription in islets of Langerhans.  |  Pedersen, AA., et al. 2002. Biochem Biophys Res Commun. 295: 243-8. PMID: 12150938
4. Overexpression of glutamic acid decarboxylase-67 (GAD-67) in gonadotropin-releasing hormone neurons disrupts migratory fate and female reproductive function in mice.  |  Heger, S., et al. 2003. Endocrinology. 144: 2566-79. PMID: 12746320
5. Glutamic acid decarboxylase (GAD)67, but not GAD65, is constitutively expressed during development and transiently overexpressed by activity in the granule cells of the rat.  |  Maqueda, J., et al. 2003. Neurosci Lett. 353: 69-71. PMID: 14642440
6. Stereological quantification of GAD-67-immunoreactive neurons and boutons in the hippocampus of middle-aged and old Fischer 344 x Brown Norway rats.  |  Shi, L., et al. 2004. J Comp Neurol. 478: 282-91. PMID: 15368530
7. Effect of honokiol on activity of GAD(65) and GAD(67) in the cortex and hippocampus of mice.  |  Ku, TH., et al. 2011. Phytomedicine. 18: 1126-9. PMID: 21561750
8. Influence of the dose of ketamine used on schizophrenia-like symptoms in mice: A correlation study with TH, GAD67, and PPAR-γ.  |  Rodrigues, T., et al. 2023. Pharmacol Biochem Behav. 233: 173658. PMID: 37804866
9. Effects of Acute Haloperidol Treatment on Dopaminergic Markers, GAD67, and A2A Receptors in Rats with High and Low VCMs.  |  Bressan, GN., et al. 2024. Neurochem Res. 50: 4. PMID: 39540951
10. Ketamine impairs the performance of male mice in novel recognition object test and reduces the immunoreactivity of GAD67 in the hippocampus: Role of pioglitazone.  |  Rodrigues, T., et al. 2024. Pharmacol Biochem Behav. 247: 173950. PMID: 39725040

The preferred GAD-67 antibody is GAD-67 Antibody (F-6): sc-28376.