GAD-67 Antibody (F-6): sc-28376

GAD-67 Antibody (F-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects GAD-67 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GAD-67 Antibody (F-6) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. GAD-67, also known as GAD1 or SCP, plays a crucial role in the synthesis of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the brain. GAD-67 is essential for maintaining the balance of excitatory and inhibitory signals in the central nervous system, which is vital for normal brain function and preventing neurological disorders. Unlike GAD-65, which is localized to synaptic vesicle membranes in nerve terminals and activated during increased demand for GABA, GAD-67 is distributed throughout the cell and is responsible for the basal levels of GABA synthesis. Loss of GAD-67 can lead to severe consequences, including lethality, highlighting GAD-67′s importance in neurotransmission. Additionally, GAD-67 undergoes alternative splicing, resulting in two isoforms: the predominant GAD-67 form and the minor GAD-25 form, which is not expressed in the brain but can be found in various endocrine tissues. GAD-67 antibody (F-6) is an invaluable tool for researchers studying GABAergic signaling and its implications in neurobiology.

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GAD-67 Antibody (F-6) References:

1. Differential expression of GAD(65) and GAD(67) during the development of the rat retina.  |  Dkhissi, O., et al. 2001. Brain Res. 919: 242-9. PMID: 11701136
2. Immune reactivity to GAD25 in type 1 diabetes mellitus.  |  Chessler, SD., et al. 2002. Autoimmunity. 35: 335-41. PMID: 12515288
3. Coexistence of GAD-65 and GAD-67 with tyrosine hydroxylase and nitric oxide synthase in amacrine and interplexiform cells of the primate, Cebus apella.  |  Andrade da Costa, BL. and Hokoç, JN. 2003. Vis Neurosci. 20: 153-63. PMID: 12916737
4. Expression of GAD67 and novel GAD67 splice variants during human fetal pancreas development: GAD67 expression in the fetal pancreas.  |  Korpershoek, E., et al. 2007. Endocr Pathol. 18: 31-6. PMID: 17652798
5. Comparison of glutamic acid decarboxylase 67 immunoreactive neurons in the hippocampal CA1 region at various age stages in dogs.  |  Hwang, IK., et al. 2008. Neurosci Lett. 431: 251-5. PMID: 18166269
6. Cyclophosphamide for anti-GAD antibody-positive refractory status epilepticus.  |  Kanter, IC., et al. 2008. Epilepsia. 49: 914-20. PMID: 18177354
7. Sensory deafferentation transsynaptically alters neuronal GluR1 expression in the external plexiform layer of the adult mouse main olfactory bulb.  |  Hamilton, KA., et al. 2008. Chem Senses. 33: 201-10. PMID: 18184638
8. Innocuous IFNgamma induced by adjuvant-free antigen restores normoglycemia in NOD mice through inhibition of IL-17 production.  |  Jain, R., et al. 2008. J Exp Med. 205: 207-18. PMID: 18195074
9. A palmitoylation cycle dynamically regulates partitioning of the GABA-synthesizing enzyme GAD65 between ER-Golgi and post-Golgi membranes.  |  Kanaani, J., et al. 2008. J Cell Sci. 121: 437-49. PMID: 18230651
10. Post-translational regulation of L-glutamic acid decarboxylase in the brain.  |  Wei, J. and Wu, JY. 2008. Neurochem Res. 33: 1459-65. PMID: 18270816
11. Some gamma-motoneurons contain gamma-aminobutyric acid in the rat cervical spinal cord.  |  Ito, T., et al. 2008. Brain Res. 1201: 78-87. PMID: 18294622
12. Relationship of GAD(67) regulation to cell cycle and DNA repair in GABA neurons in the adult hippocampus: bipolar disorder versus schizophrenia.  |  Benes, FM. 2010. Cell Cycle. 9: 625-7. PMID: 20107308

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAD-67 Antibody (F-6) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-GAD-67 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAD-67 Antibody (F-6) Specifications

Alternate Product Name: GAD-67

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1-101

Epitope Target: GAD-67

Epitope Species: human

Product Reactivity/Detection: GAD-67

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for GAD-67 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin