GABP-β1/2 Antibody (E-7): sc-271571
- GABP-β1/2 Antibody (E-7) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 131-395 mapping at the C-terminus of GABP-β1 of human origin
- GABP- beta 1/2 Antibody (E-7) is recommended for detection of GABP-β1 and GABP-β2 of human origin by WB, IP, IF and ELISA
- Anti-GABP- beta 1/2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271571 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GABP-β1/2 Antibody (E-7) for WB applications. These reagents are now offered in bundles with GABP-β1/2 Antibody (E-7) (see ordering information below).
GABP-β1/2 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects GABP-β1 and GABP-β2 proteins of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GABP-β1/2 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The GA-binding protein (GABP) transcription factor is crucial for regulating gene expression and is composed of two subunits: the Ets-related GABP-α and GABP-β subunit, which has two splice variants, GABP-β1 and GABP-β2, that differ in their C-termini. GABP-β is predominantly localized in both the cytoplasm and nucleus of primary neuronal cultures, while GABP-α is primarily nuclear. This localization allows GABP to coordinate transcriptional regulation between nucleus and mitochondria, particularly for genes involved in energy metabolism and cellular stress responses. The GABPαβ tetrameric complex plays a vital role in various cellular processes, including stimulation of adenovirus E4 gene transcription, differential activation of BRCA1 expression in breast cell lines, and regulation of mitochondrial and nuclear transcription for cytochrome oxidase in neurons. GABP-β1/2 (E-7) antibody enables researchers to gain insights into complex regulatory mechanisms of GABP and implications in cellular function and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GABP-β1/2 Antibody (E-7) References:
- Synergistic transcriptional activation by hGABP and select members of the activation transcription factor/cAMP response element-binding protein family. | Sawada, J., et al. 1999. J Biol Chem. 274: 35475-82. PMID: 10585419
- The alpha and beta subunits of the GA-binding protein form a stable heterodimer in solution. Revised model of heterotetrameric complex assembly. | Chinenov, Y., et al. 2000. J Biol Chem. 275: 7749-56. PMID: 10713087
- Depolarizing stimulation upregulates GA-binding protein in neurons: a transcription factor involved in the bigenomic expression of cytochrome oxidase subunits. | Zhang, C. and Wong-Riley, MT. 2000. Eur J Neurosci. 12: 1013-23. PMID: 10762332
- GA-binding protein alpha/beta is a critical regulator of the BRCA1 promoter. | Atlas, E., et al. 2000. Oncogene. 19: 1933-40. PMID: 10773883
- Functional interactions of transcription factor human GA-binding protein subunits. | Suzuki, F., et al. 1998. J Biol Chem. 273: 29302-8. PMID: 9792629
- Evolution of the human immunodeficiency virus type 1 long terminal repeat promoter by conversion of an NF-kappaB enhancer element into a GABP binding site. | Verhoef, K., et al. 1999. J Virol. 73: 1331-40. PMID: 9882338
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GABP-β1/2 Antibody (E-7) | sc-271571 | 200 µg/ml | $322.00 | |||
GABP-β1/2 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-529212 | 200 µg Ab; 10 µg BP | $361.00 | |||
GABP-β1/2 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-521983 | 200 µg Ab, 40 µg BP | $361.00 | |||
GABP-β1/2 Antibody (E-7): m-IgG2a BP-HRP Bundle | sc-547279 | 200 µg Ab; 10 µg BP | $361.00 | |||
GABP-β1/2 Antibody (E-7) X | sc-271571 X | 200 µg/0.1 ml | $322.00 | |||
GABP-β1/2 Antibody (E-7) AC | sc-271571 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GABP-β1/2 Antibody (E-7) HRP | sc-271571 HRP | 200 µg/ml | $322.00 | |||
GABP-β1/2 Antibody (E-7) FITC | sc-271571 FITC | 200 µg/ml | $336.00 | |||
GABP-β1/2 Antibody (E-7) PE | sc-271571 PE | 200 µg/ml | $349.00 | |||
GABP-β1/2 Antibody (E-7) Alexa Fluor® 488 | sc-271571 AF488 | 200 µg/ml | $364.00 | |||
GABP-β1/2 Antibody (E-7) Alexa Fluor® 546 | sc-271571 AF546 | 200 µg/ml | $364.00 | |||
GABP-β1/2 Antibody (E-7) Alexa Fluor® 594 | sc-271571 AF594 | 200 µg/ml | $364.00 | |||
GABP-β1/2 Antibody (E-7) Alexa Fluor® 647 | sc-271571 AF647 | 200 µg/ml | $364.00 | |||
GABP-β1/2 Antibody (E-7) Alexa Fluor® 680 | sc-271571 AF680 | 200 µg/ml | $364.00 | |||
GABP-β1/2 Antibody (E-7) Alexa Fluor® 790 | sc-271571 AF790 | 200 µg/ml | $364.00 |