Anti-GABP- beta 1/2 Antibody (E-7) recommended for detection of GABP-β1 and GABP-β2 of human origin by WB, IP, IF and ELISA

Anti-GABP-β1/2 Antibody (E-7): sc-271571

Anti-GABP-β1/2 Antibody (E-7) is rated 5.0 out of 5 by 3.
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Datasheets

    • GABP-β1/2 Antibody (E-7) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 131-395 mapping at the C-terminus of GABP-β1 of human origin
    • GABP- beta 1/2 Antibody (E-7) is recommended for detection of GABP-β1 and GABP-β2 of human origin by WB, IP, IF and ELISA
    • Anti-GABP- beta 1/2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-271571 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GABP-β1/2 (E-7): sc-271571.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for GABP-β1/2 Antibody (E-7) for WB applications. These reagents are now offered in bundles with GABP-β1/2 Antibody (E-7) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with GABP-β1/2 (E-7): sc-271571 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Working well for WB in HeLa cells Working well for WB in HeLa cells.
Date published: 2015-06-18
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear staining Beautiful immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-22
Rated 5 out of 5 by from Produced positive Western Blot data of GABP Produced positive Western Blot data of GABP- 1/2 expression in Jurkat whole cell lysate and HeLa nuclear extract . -SCBT QC
Date published: 2014-12-14
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