Gab 3 Antibody (E-1): sc-271475

Gab 3 Antibody (E-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gab 3 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gab 3 (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Gab (GRB2-associated binder)/DOS (′Daughter of Sevenless′) family of adaptor proteins, which includes Gab 3, plays a pivotal role as molecular scaffolds that facilitate protein recruitment to receptor tyrosine kinases (RTKs). Such recruitment is essential for propagating signaling cascades initiated by cytokine and growth factor interactions with RTKs, ultimately leading to cellular responses such as activation, proliferation, and differentiation. Gab 3 structure contains pleckstrin homology domains and potential binding sites for SH2 and SH3 domain-containing proteins, which are crucial for phosphorylation-dependent recruitment of signaling partners. The human Gab3 gene, located on chromosome Xq28, encodes a 586 amino acid protein that is integral to insulin receptor and epidermal growth factor receptor signaling pathways, underscoring its significance in mediating cellular responses to extracellular stimuli.

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Gab 3 Antibody (E-1) References:

1. Gab2, a new pleckstrin homology domain-containing adapter protein, acts to uncouple signaling from ERK kinase to Elk-1.  |  Zhao, C., et al. 1999. J Biol Chem. 274: 19649-54. PMID: 10391903
2. Identification of an atypical Grb2 carboxyl-terminal SH3 domain binding site in Gab docking proteins reveals Grb2-dependent and -independent recruitment of Gab1 to receptor tyrosine kinases.  |  Lock, LS., et al. 2000. J Biol Chem. 275: 31536-45. PMID: 10913131
3. Gab3, a new DOS/Gab family member, facilitates macrophage differentiation.  |  Wolf, I., et al. 2002. Mol Cell Biol. 22: 231-44. PMID: 11739737
4. Alternative pathway of insulin signalling in mice with targeted disruption of the IRS-1 gene.  |  Araki, E., et al. 1994. Nature. 372: 186-90. PMID: 7526222
5. A Grb2-associated docking protein in EGF- and insulin-receptor signalling.  |  Holgado-Madruga, M., et al. 1996. Nature. 379: 560-4. PMID: 8596638

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gab 3 Antibody (E-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Gab 3 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gab 3 Antibody (E-1) Specifications

Alternate Product Name: Gab 3

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 101-400

Epitope Target: Gab 3

Epitope Species: human

Product Reactivity/Detection: Gab 3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Gab 3 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin