G2A Antibody (G-5): sc-137112

G2A Antibody (G-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects G2A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). G2A (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G2A, also known as G Protein-Coupled Receptor 132, plays a crucial role as a seven transmembrane G protein-coupled receptor that is upregulated in response to DNA damage and cellular stress, highlighting its importance in cellular signaling pathways that govern cell survival and proliferation. G2A is predominantly expressed in hematopoietic tissues, particularly in hematopoietic stem cells and pro-B cells, with lower levels found in immature B cells and pre-B cells. Expression is also observed throughout T cell maturation and significantly increases upon T-cell activation, indicating involvement in immune responses. Notably, ectopic expression of G2A fusion protein in NIH/3T3 fibroblasts induces cell cycle arrest at the G2/M transition, suggesting a regulatory role in cell cycle progression. G2A has been implicated in anti-oncogenic activity, as G2A can attenuate the proliferative effects of the Bcr-Abl oncogene, which is associated with certain leukemias. G2A contains a destruction box motif, essential for ubiquitination and subsequent proteolytic degradation, a feature commonly found in cyclins that regulates cell cycle dynamics. As a high-affinity receptor for lysophosphatidylcholine, G2A is part of the lysophospholipid receptor subfamily, underscoring its significance in lipid signaling and cellular communication.

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G2A Antibody (G-5) References:

1. G2A is an oncogenic G protein-coupled receptor.  |  Zohn, IE., et al. 2000. Oncogene. 19: 3866-77. PMID: 10951580
2. Lysophosphatidylcholine as a ligand for the immunoregulatory receptor G2A.  |  Kabarowski, JH., et al. 2001. Science. 293: 702-5. PMID: 11474113
3. Expression of G2A, a receptor for lysophosphatidylcholine, by macrophages in murine, rabbit, and human atherosclerotic plaques.  |  Rikitake, Y., et al. 2002. Arterioscler Thromb Vasc Biol. 22: 2049-53. PMID: 12482833
4. The lysophospholipid receptor G2A activates a specific combination of G proteins and promotes apoptosis.  |  Lin, P. and Ye, RD. 2003. J Biol Chem. 278: 14379-86. PMID: 12586833
5. G2A plays proinflammatory roles in human keratinocytes under oxidative stress as a receptor for 9-hydroxyoctadecadienoic acid.  |  Hattori, T., et al. 2008. J Invest Dermatol. 128: 1123-33. PMID: 18034171
6. G2A as a receptor for oxidized free fatty acids.  |  Obinata, H. and Izumi, T. 2009. Prostaglandins Other Lipid Mediat. 89: 66-72. PMID: 19063986
7. G2A and LPC: regulatory functions in immunity.  |  Kabarowski, JH. 2009. Prostaglandins Other Lipid Mediat. 89: 73-81. PMID: 19383550
8. Identification and analysis of two splice variants of human G2A generated by alternative splicing.  |  Ogawa, A., et al. 2010. J Pharmacol Exp Ther. 332: 469-78. PMID: 19855098
9. The Lipid Receptor G2A (GPR132) Mediates Macrophage Migration in Nerve Injury-Induced Neuropathic Pain.  |  Osthues, T., et al. 2020. Cells. 9: PMID: 32708184
10. Elucidating the biological characteristics and pathogenicity of the highly virulent G2a porcine epidemic diarrhea virus.  |  Li, C., et al. 2024. J Gen Virol. 105: PMID: 38270573
11. A DNA damage and stress inducible G protein-coupled receptor blocks cells in G2/M.  |  Weng, Z., et al. 1998. Proc Natl Acad Sci U S A. 95: 12334-9. PMID: 9770487

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G2A Antibody (G-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-G2A Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G2A Antibody (G-5) Specifications

Alternate Product Name: GPR132

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 311-380

Epitope Target: G2A

Epitope Species: human

Product Reactivity/Detection: G2A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for G2A Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin