Furin Antibody (B-6): sc-133142

Furin Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Furin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Furin (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Furin (FUR, PACE, PCSK3, SPC1, Kex2p) is a calcium-dependent serine endoprotease that plays a crucial role in processing latent precursor proteins into active forms, essential for numerous physiological processes. Located primarily in the trans-Golgi network, Furin is synthesized as an inactive zymogen, a mechanism preventing premature activation and potential protein degradation. This zymogen form contains an inactivating prosegment at its N-terminal, which remains associated until reaching the trans-Golgi network, where autocatalytic cleavage occurs, leading to prosegment release and Furin activation. Furin′s ability to cleave at paired basic amino acid processing sites is vital for activating several important proteins, including proparathyroid hormone and transforming growth factor beta 1 precursor, highlighting significance in cellular signaling and homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Furin Antibody (B-6) References:

1. Furin inhibition by compounds of copper and zinc.  |  Podsiadlo, P., et al. 2004. J Biol Chem. 279: 36219-27. PMID: 15140896
2. Plasticity of extended subsites facilitates divergent substrate recognition by Kex2 and furin.  |  Rozan, L., et al. 2004. J Biol Chem. 279: 35656-63. PMID: 15159396
3. Beta-amyloid protein converting enzyme 1 and brain-specific type II membrane protein BRI3: binding partners processed by furin.  |  Wickham, L., et al. 2005. J Neurochem. 92: 93-102. PMID: 15606899
4. Furin directly cleaves proMMP-2 in the trans-Golgi network resulting in a nonfunctioning proteinase.  |  Cao, J., et al. 2005. J Biol Chem. 280: 10974-80. PMID: 15637056
5. Human carcinoma cell growth and invasiveness is impaired by the propeptide of the ubiquitous proprotein convertase furin.  |  López de Cicco, R., et al. 2005. Cancer Res. 65: 4162-71. PMID: 15899807
6. Structure and expression of mouse furin, a yeast Kex2-related protease. Lack of processing of coexpressed prorenin in GH4C1 cells.  |  Hatsuzawa, K., et al. 1990. J Biol Chem. 265: 22075-8. PMID: 2266110
7. Furin Protease: From SARS CoV-2 to Anthrax, Diabetes, and Hypertension.  |  Fitzgerald, K. 2020. Perm J. 24: PMID: 33202215
8. Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis.  |  Johnson, BA., et al. 2021. Nature. 591: 293-299. PMID: 33494095
9. The Path to Therapeutic Furin Inhibitors: From Yeast Pheromones to SARS-CoV-2.  |  Thomas, G., et al. 2022. Int J Mol Sci. 23: PMID: 35408793
10. Furin as a therapeutic target in cystic fibrosis airways disease.  |  Douglas, LEJ., et al. 2023. Eur Respir Rev. 32: PMID: 37137509
11. Targeting furin, a cellular proprotein convertase, for COVID-19 prevention and therapeutics.  |  Jiang, X., et al. 2024. Drug Discov Today. 29: 104026. PMID: 38762086
12. Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins.  |  Nakayama, K. 1997. Biochem J. 327 (Pt 3): 625-35. PMID: 9599222

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Furin Antibody (B-6) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-Furin Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Furin Antibody (B-6) Specifications

Alternate Product Name: Furin, Membrane Associated Receptor Protein

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 575-794

Epitope Target: Furin

Epitope Species: human

Product Reactivity/Detection: Furin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Furin Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Furin antibody is Furin Antibody (G-6): sc-133141.