frizzled-4 Antibody (3G7): sc-293454

frizzled-4 Antibody (3G7) is a mouse monoclonal IgG2b kappa light chain antibody that detects frizzled-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-frizzled-4 antibody (3G7) is available as a non-conjugated format. Frizzled-4 protein, consisting of 537 amino acids and encoded by the human gene FZD4, serves as a crucial receptor for Wnt proteins, which are essential for various developmental processes and cellular functions. Frizzled-4 (3G7) monoclonal antibody plays an integral role in the beta-catenin canonical signaling pathway, which facilitates the activation of disheveled proteins, inhibits glycogen synthase kinase 3, promotes the nuclear accumulation of beta-catenin, and ultimately activates Wnt target genes. This signaling pathway regulates cell proliferation, differentiation, and tissue homeostasis. Frizzled-4 is implicated in retinal angiogenesis, highlighting a significant role in vascular development and potential implications in diseases such as diabetic retinopathy. Expression of frizzled-4 is widespread, with highest levels found in adult heart, skeletal muscle, ovary, and fetal kidney, underscoring a role in various physiological processes.

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frizzled-4 Antibody (3G7) References:

1. Mutant frizzled-4 disrupts retinal angiogenesis in familial exudative vitreoretinopathy.  |  Robitaille, J., et al. 2002. Nat Genet. 32: 326-30. PMID: 12172548
2. Autosomal dominant familial exudative vitreoretinopathy in two Japanese families with FZD4 mutations (H69Y and C181R).  |  Omoto, S., et al. 2004. Ophthalmic Genet. 25: 81-90. PMID: 15370539
3. Genetic variants of frizzled-4 gene in familial exudative vitreoretinopathy and advanced retinopathy of prematurity.  |  MacDonald, ML., et al. 2005. Clin Genet. 67: 363-6. PMID: 15733276
4. Complexity of the genotype-phenotype correlation in familial exudative vitreoretinopathy with mutations in the LRP5 and/or FZD4 genes.  |  Qin, M., et al. 2005. Hum Mutat. 26: 104-12. PMID: 15981244
5. Wnt/beta-catenin signaling induces proliferation, survival and interleukin-8 in human endothelial cells.  |  Masckauchán, TN., et al. 2005. Angiogenesis. 8: 43-51. PMID: 16132617
6. Xenopus frizzled-4S, a splicing variant of Xfz4 is a context-dependent activator and inhibitor of Wnt/beta-catenin signaling.  |  Swain, RK., et al. 2005. Cell Commun Signal. 3: 12. PMID: 16236168
7. Purified Wnt5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context.  |  Mikels, AJ. and Nusse, R. 2006. PLoS Biol. 4: e115. PMID: 16602827
8. Wnt5a promotes Frizzled-4 signalosome assembly by stabilizing cysteine-rich domain dimerization.  |  DeBruine, ZJ., et al. 2017. Genes Dev. 31: 916-926. PMID: 28546512
9. Wnt Receptor Frizzled-4 as a Marker for Isolation of Enteric Neural Progenitors in Human Children.  |  Neckel, PH., et al. 2019. Cells. 8: PMID: 31366044
10. Frizzled-4 is required for normal bone acquisition despite compensation by Frizzled-8.  |  Kushwaha, P., et al. 2020. J Cell Physiol. 235: 6673-6683. PMID: 31985040
11. Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway.  |  Rickman, M., et al. 2023. J Cell Sci. 136: PMID: 36846872
12. Structural basis of Frizzled 4 in recognition of Dishevelled 2 unveils mechanism of WNT signaling activation.  |  Qian, Y., et al. 2024. Nat Commun. 15: 7644. PMID: 39223191