FOXP3 Antibody (2A11G9): sc-53876

FOXP3 Antibody (2A11G9) is a mouse monoclonal IgG2a kappa light chain antibody that detects the FOXP3 protein of mouse, rat, and human origin by immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). FOXP3 (2A11G9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FOXP3, also known as scurfin, plays a critical role in maintaining immune homeostasis by acting as a transcriptional repressor. FOXP3 is essential for the development and function of regulatory T cells, which are crucial for preventing autoimmune diseases and maintaining tolerance to self-antigens. Mutations in the FOXP3 gene can lead to immune dysregulation, resulting in the severe X-linked disorder known as immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX). FOXP3′s ability to bind DNA through its forkhead domain allows regulation of genes involved in T cell activation and differentiation, highlighting FOXP3′s importance in the immune response. The gene encoding FOXP3 is located on the X chromosome at position Xp11.23, and FOXP3 expression is tightly regulated during T cell development, making FOXP3 a vital marker for identifying and studying regulatory T cells in various research and clinical applications.

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FOXP3 Antibody (2A11G9) References:

1. The SPCH1 region on human 7q31: genomic characterization of the critical interval and localization of translocations associated with speech and language disorder.  |  Lai, CS., et al. 2000. Am J Hum Genet. 67: 357-68. PMID: 10880297
2. The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3.  |  Bennett, CL., et al. 2001. Nat Genet. 27: 20-1. PMID: 11137993
3. Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse.  |  Brunkow, ME., et al. 2001. Nat Genet. 27: 68-73. PMID: 11138001
4. Characterization of a new subfamily of winged-helix/forkhead (Fox) genes that are expressed in the lung and act as transcriptional repressors.  |  Shu, W., et al. 2001. J Biol Chem. 276: 27488-97. PMID: 11358962
5. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation.  |  Schubert, LA., et al. 2001. J Biol Chem. 276: 37672-9. PMID: 11483607
6. The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p.  |  Banham, AH., et al. 2001. Cancer Res. 61: 8820-9. PMID: 11751404
7. FOXP3 and its role in the immune system.  |  Kim, CH. 2009. Adv Exp Med Biol. 665: 17-29. PMID: 20429413
8. Two FOXP3(+)CD4(+) T cell subpopulations distinctly control the prognosis of colorectal cancers.  |  Saito, T., et al. 2016. Nat Med. 22: 679-84. PMID: 27111280
9. Human FOXP3+ Regulatory T Cell Heterogeneity and Function in Autoimmunity and Cancer.  |  Wing, JB., et al. 2019. Immunity. 50: 302-316. PMID: 30784578
10. Human FOXP3 and tumour microenvironment.  |  Wang, J., et al. 2023. Immunology. 168: 248-255. PMID: 35689826
11. Genetic tracing reveals transcription factor Foxp3-dependent and Foxp3-independent functionality of peripherally induced Treg cells.  |  van der Veeken, J., et al. 2022. Immunity. 55: 1173-1184.e7. PMID: 35700740
12. FOXP3 recognizes microsatellites and bridges DNA through multimerization.  |  Zhang, W., et al. 2023. Nature. 624: 433-441. PMID: 38030726

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FOXP3 Antibody (2A11G9) has 40 citations in a variety of scientific publications.

Primary antibodies like Anti-FOXP3 Antibody (2A11G9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FOXP3 Antibody (2A11G9) Specifications

Alternate Product Name: forkhead box P3 (FOXP3)

Product Clone Name: 2A11G9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: FOXP3

Epitope Species: human

Product Reactivity/Detection: FOXP3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 40 citations in various scientific publications

Available Conjugates/Forms for FOXP3 Antibody (2A11G9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin