FOXM1 Antibody (E-8): sc-376620

FOXM1 Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect FOXM1 across mouse, rat, and human species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FOXM1 monoclonal antibody (E-8) targets a precise epitope mapping between amino acids 735-763 at the C-terminus of human FOXM1, ensuring robust and reliable binding for accurate detection and analysis. Forkhead box M1 (FOXM1), also called FKHL16, HFH11, INS-1, and PIG29, is a critical transcription factor involved in the regulation of cell proliferation, cell cycle progression, and DNA damage repair, playing a pivotal role in the transition from G1 to S phase and mitosis by activating key cell cycle regulators like cyclin B1, cyclin D1, and Cdc25B. The structure of FOXM1, including its distinct forkhead DNA-binding domain and the C-terminal region targeted by anti-FOXM1 antibody (E-8), is essential for interaction with DNA and other regulatory proteins, facilitating its role in controlling gene expression. FOXM1 undergoes several post-translational modifications, including phosphorylation, ubiquitination, and acetylation, which are critical for stability, subcellular localization, and transcriptional activity. Phosphorylation of FOXM1 by kinases such as CDKs and PLK1 enhances transcriptional activity and promotes nuclear localization, while ubiquitination regulates proteasomal degradation, ensuring tight control over cellular levels. Acetylation of FOXM1 also plays a role in modulating activity and interactions with other proteins. These post-translational modifications are crucial for FOXM1′s function in normal cellular processes and dysregulation is often implicated in cancer, where overactive FOXM1 can lead to uncontrolled cell proliferation and tumor progression. Alternative splicing of FOXM1 results in isoforms such as FOXM1A and FOXM1B, which have distinct roles in protein stability and function, further highlighting the importance of post-translational modifications in modulating FOXM1 activity. FOXM1 (E-8) antibody is available in both non-conjugated form and various conjugated forms, providing versatility for diverse experimental needs.

The preferred FOXM1 antibody is FOXM1 Antibody (A-11): sc-271746.