Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FOXL2 CRISPR/Cas9 KO Plasmid (h): sc-403351
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXL2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FOXL2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXL2 Antibody (262C1a): sc-81275
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXL2 CRISPR/Cas9 KO Plasmid (h) | sc-403351 | 20 µg | $397.00 |
Overview
FOXL2 is a forkhead box transcription factor that plays a central role in granulosa cell differentiation, ovarian folliculogenesis, and maintenance of ovarian identity. By binding cis-regulatory elements and coordinating chromatin-associated transcriptional programs, FOXL2 integrates signaling inputs that shape steroidogenesis, cell-cycle control, and apoptosis in reproductive tissues. Dysregulated FOXL2 activity perturbs developmental gene networks and is strongly linked to disorders of sex development and ovarian pathobiology, including recurrent somatic alterations observed in adult-type granulosa cell tumors. As a nuclear regulator with lineage-defining functions, FOXL2 is widely studied in pathways governing reproductive endocrinology, transcriptional regulation, and tissue-specific tumor biology.
FOXL2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FOXL2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FOXL2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FOXL2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FOXL2 protein expression.
This CRISPR knockout system enables efficient generation of FOXL2-deficient cell models for investigation of FOXL2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FOXL2 exon(s) critical for FOXL2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FOXL2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FOXL2 CRISPR/Cas9 KO Plasmid (h) and FOXL2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FOXL2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FOXL2 HDR Plasmid (h) and FOXL2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FOXL2 homology arms to support homology-directed repair at defined FOXL2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.