Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FOXC2 CRISPR/Cas9 KO Plasmid (m): sc-420370
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FOXC2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FOXC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FOXC2 Antibody (G-7): sc-515234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FOXC2 CRISPR/Cas9 KO Plasmid (m) | sc-420370 | 20 µg | $397.00 |
Overview
Foxc2 encodes the forkhead box transcription factor FOXC2, a DNA-binding regulator that coordinates gene expression programs controlling mesenchymal cell identity, vascular and lymphatic development, and adipose tissue remodeling in mouse. FOXC2 functions within nucleus-driven transcriptional networks that intersect with developmental signaling pathways such as TGF-β and Notch to influence endothelial differentiation, extracellular matrix organization, and epithelial–mesenchymal transition–like processes. Altered FOXC2 activity has been linked to dysregulated lymphangiogenesis, aberrant vascular patterning, and shifts in metabolic and stromal states that are commonly investigated in models of developmental disorders and tumor microenvironment biology. These roles make Foxc2 a useful node for dissecting transcriptional control of tissue morphogenesis and cell fate decisions.
FOXC2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Foxc2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Foxc2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Foxc2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FOXC2 protein expression.
This CRISPR knockout system enables efficient generation of Foxc2-deficient cell models for investigation of FOXC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Foxc2 exon(s) critical for FOXC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Foxc2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FOXC2 CRISPR/Cas9 KO Plasmid (m) and FOXC2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Foxc2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FOXC2 HDR Plasmid (m) and FOXC2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Foxc2 homology arms to support homology-directed repair at defined Foxc2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.