FOG-2 Antibody (H-5): sc-398011

FOG-2 Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects FOG-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FOG-2 (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FOG-2, a member of the zinc finger protein family, plays a crucial role in regulating gene expression during development, particularly in hematopoiesis and cardiac function. FOG-2 interacts with GATA transcription factors, forming complexes essential for the maturation of erythroid and megakaryocyte lineages. FOG-2 is predominantly expressed in the heart, neurons, and gonads, where FOG-2 exhibits a dual regulatory role; FOG-2 can enhance or inhibit GATA-mediated transcription depending on the cellular context. In cardiomyocytes and fibroblasts, FOG-2 inhibits GATA-4 transcriptional activity, while FOG-2 can restore GATA-1 mediated transcription in erythroid cultures lacking FOG, highlighting FOG-2′s importance in fine-tuning transcriptional responses during development and differentiation. This versatility underscores FOG-2′s significance in both normal physiological processes and potential pathological conditions, making anti-FOG-2 antibody (H-5) a valuable tool for researchers studying gene regulation and cellular differentiation.

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FOG-2 Antibody (H-5) References:

1. Transcriptional cofactors of the FOG family interact with GATA proteins by means of multiple zinc fingers.  |  Fox, AH., et al. 1999. EMBO J. 18: 2812-22. PMID: 10329627
2. FOG-2, a heart- and brain-enriched cofactor for GATA transcription factors.  |  Lu, JR., et al. 1999. Mol Cell Biol. 19: 4495-502. PMID: 10330188
3. FOG-2 competes with GATA-4 for transcriptional coactivator p300 and represses hypertrophic responses in cardiac myocytes.  |  Hirai, M., et al. 2004. J Biol Chem. 279: 37640-50. PMID: 15220332
4. FOG-1 represses GATA-1-dependent FcepsilonRI beta-chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice.  |  Maeda, K., et al. 2006. Blood. 108: 262-9. PMID: 16522818
5. Disruption of friend of GATA 2 gene (FOG-2) by a de novo t(8;10) chromosomal translocation is associated with heart defects and gonadal dysgenesis.  |  Finelli, P., et al. 2007. Clin Genet. 71: 195-204. PMID: 17309641
6. FOG-2 mediated recruitment of the NuRD complex regulates cardiomyocyte proliferation during heart development.  |  Garnatz, AS., et al. 2014. Dev Biol. 395: 50-61. PMID: 25196150
7. The LXCXE Retinoblastoma Protein-Binding Motif of FOG-2 Regulates Adipogenesis.  |  Goupille, O., et al. 2017. Cell Rep. 21: 3524-3535. PMID: 29262331
8. The integrity of the FOG-2 LXCXE pRb-binding motif is required for small intestine homeostasis.  |  Goupille, O., et al. 2019. Exp Physiol. 104: 1074-1089. PMID: 31012180
9. FOG, a multitype zinc finger protein, acts as a cofactor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation.  |  Tsang, AP., et al. 1997. Cell. 90: 109-19. PMID: 9230307
10. Failure of megakaryopoiesis and arrested erythropoiesis in mice lacking the GATA-1 transcriptional cofactor FOG.  |  Tsang, AP., et al. 1998. Genes Dev. 12: 1176-88. PMID: 9553047
11. FOG-2: A novel GATA-family cofactor related to multitype zinc-finger proteins Friend of GATA-1 and U-shaped.  |  Tevosian, SG., et al. 1999. Proc Natl Acad Sci U S A. 96: 950-5. PMID: 9927674
12. Molecular cloning of FOG-2: a modulator of transcription factor GATA-4 in cardiomyocytes.  |  Svensson, EC., et al. 1999. Proc Natl Acad Sci U S A. 96: 956-61. PMID: 9927675

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FOG-2 Antibody (H-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-FOG-2 Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FOG-2 Antibody (H-5) Specifications

Alternate Product Name: ZFPM2

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 880-1126

Epitope Target: FOG-2

Epitope Species: mouse

Product Reactivity/Detection: FOG-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for FOG-2 Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent