Flt-4 Antibody (E-3): sc-514825

Flt-4 Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Flt-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Flt-4 antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Flt-4, also known as vascular endothelial growth factor receptor 3 (VEGFR-3), plays a crucial role in lymphangiogenesis and lymphatic system development, which is essential for maintaining fluid homeostasis and immune responses. Flt-4 is primarily located on endothelial cell surfaces, particularly in lymphatic vessels, where Flt-4 mediates vascular endothelial growth factors (VEGFs) effects and regulates lymphatic endothelial cell proliferation and migration. Proper Flt-4 functioning is vital for new lymphatic vessel formation and lymphatic integrity maintenance, making Flt-4 a significant target for research in cancer metastasis and inflammatory diseases. Flt-4 interacts with VEGF-C and VEGF-D, which are key ligands that activate the receptor, further underscoring Flt-4′s importance in vascular biology and potential therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Flt-4 Antibody (E-3) References:

1. Vegf, Vegf-B, Vegf-C and their receptors KDR, FLT-1 and FLT-4 during the neoplastic progression of human colonic mucosa.  |  André, T., et al. 2000. Int J Cancer. 86: 174-81. PMID: 10738243
2. FLT4 receptor tyrosine kinase contains seven immunoglobulin-like loops and is expressed in multiple human tissues and cell lines.  |  Pajusola, K., et al. 1992. Cancer Res. 52: 5738-43. PMID: 1327515
3. Flt-4-positive endothelial cell density and its clinical significance in non-small cell lung cancer.  |  Chen, F., et al. 2004. Clin Cancer Res. 10: 8548-53. PMID: 15623638
4. VEGF-C, VEGF-D and Flt-4 in transitional bladder cancer: relationships to clinicopathological parameters and long-term survival.  |  Herrmann, E., et al. 2007. Anticancer Res. 27: 3127-33. PMID: 17970053
5. Fms-like tyrosine kinase (Flt)-4 signaling participates in osteoclast differentiation in osteopetrotic (op/op) mice.  |  Tsuka, N., et al. 2009. Biomed Res. 30: 31-7. PMID: 19265261
6. Genistein suppresses FLT4 and inhibits human colorectal cancer metastasis.  |  Xiao, X., et al. 2015. Oncotarget. 6: 3225-39. PMID: 25605009
7. FOXC2 and FLT4 Gene Variants in Lymphatic Filariasis.  |  Sheik, Y., et al. 2015. Lymphat Res Biol. 13: 112-9. PMID: 26091406
8. Surface Engineering of FLT4-Targeted Nanocarriers Enhances Cell-Softening Glaucoma Therapy.  |  Vincent, MP., et al. 2021. ACS Appl Mater Interfaces. 13: 32823-32836. PMID: 34232612
9. Signalling properties of FLT4, a proteolytically processed receptor tyrosine kinase related to two VEGF receptors.  |  Pajusola, K., et al. 1994. Oncogene. 9: 3545-55. PMID: 7970715
10. Expression of FLT4 and its ligand VEGF-C in acute myeloid leukemia.  |  Fielder, W., et al. 1997. Leukemia. 11: 1234-7. PMID: 9264375

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Flt-4 Antibody (E-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Flt-4 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Flt-4 Antibody (E-3) Specifications

Alternate Product Name: VEGFR3 receptor tyrosine kinase

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1278-1299

Epitope Target: Flt-4

Epitope Species: mouse

Product Reactivity/Detection: Flt-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Flt-4 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Flt-4 antibody is Flt-4 Antibody (D-6): sc-28297.