Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FLJ23356 CRISPR/Cas9 KO Plasmid (h): sc-406964
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FLJ23356 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FLJ23356 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FLJ23356 Antibody (S-23): sc-100433
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FLJ23356 CRISPR/Cas9 KO Plasmid (h) | sc-406964 | 20 µg | $397.00 |
Overview
POMK (protein O-mannose kinase), also referenced as FLJ23356, encodes a Golgi-localized kinase required for phosphorylation of O-mannose on α-dystroglycan, a key step in glycan maturation that supports extracellular matrix binding. This activity functions within the O-mannosylation and dystroglycanopathy-related glycosylation network, intersecting with pathways governing basement membrane organization, cell adhesion, and muscle–brain tissue integrity. Disruption of POMK-dependent glycosylation impairs α-dystroglycan ligand interactions and perturbs membrane-associated signaling complexes. Genetic defects in POMK are associated with dystroglycanopathies and congenital muscular dystrophy phenotypes, making it a useful locus for modeling glycosylation-linked disease mechanisms.
FLJ23356 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the POMK gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the POMK together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the POMK open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FLJ23356 protein expression.
This CRISPR knockout system enables efficient generation of POMK-deficient cell models for investigation of FLJ23356 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting POMK exon(s) critical for FLJ23356 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple POMK genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FLJ23356 CRISPR/Cas9 KO Plasmid (h) and FLJ23356 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the POMK locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FLJ23356 HDR Plasmid (h) and FLJ23356 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by POMK homology arms to support homology-directed repair at defined POMK target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.