Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FLAD1 CRISPR/Cas9 KO Plasmid (h): sc-406725
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FLAD1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FLAD1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FLAD1 Antibody (G-4): sc-376819
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FLAD1 CRISPR/Cas9 KO Plasmid (h) | sc-406725 | 20 µg | $397.00 |
Overview
FLAD1 encodes flavin adenine dinucleotide synthetase 1, a bifunctional enzyme that catalyzes the adenylation of FMN to generate FAD, a key redox cofactor required by numerous mitochondrial and cytosolic flavoproteins. By controlling cellular FAD availability, FLAD1 supports oxidative metabolism, electron transfer reactions, fatty acid β-oxidation, and broader mitochondrial homeostasis, linking micronutrient-derived riboflavin metabolism to energy production and redox balance. Perturbation of FAD synthesis can disrupt flavoprotein-dependent pathways, alter reactive oxygen species handling, and compromise bioenergetic capacity. Human genetic and functional evidence connects FLAD1 dysfunction with inborn errors of flavin metabolism and mitochondrial disease phenotypes, making it relevant to studies of metabolic stress and mitochondrial biology.
FLAD1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FLAD1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FLAD1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FLAD1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FLAD1 protein expression.
This CRISPR knockout system enables efficient generation of FLAD1-deficient cell models for investigation of FLAD1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FLAD1 exon(s) critical for FLAD1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FLAD1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FLAD1 CRISPR/Cas9 KO Plasmid (h) and FLAD1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FLAD1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FLAD1 HDR Plasmid (h) and FLAD1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FLAD1 homology arms to support homology-directed repair at defined FLAD1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.