FLAD1 Antibody (G-4): sc-376819

FLAD1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects FLAD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FLAD1 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FLAD1 protein, also known as FAD1, FADS, PP591, or molybdenum cofactor biosynthesis protein-like, consists of 587 amino acids and features distinct functional domains; its N-terminus is part of the moaB/mog family, while the C-terminus belongs to the PAPS reductase family. FLAD1 plays a crucial role in converting riboflavin into flavin adenine dinucleotide (FAD), a vital redox cofactor involved in numerous biological processes, including cellular respiration and energy production. FLAD1 is predominantly localized in the cytoplasm and requires magnesium as a cofactor for enzymatic activity. FLAD1 exists in five alternatively spliced isoforms, and FLAD1 gene is located on human chromosome 1, which spans 260 million base pairs and contains over 3,000 genes, accounting for nearly 8% of the human genome. Aberrations in chromosome 1 have been implicated in various cancers, such as head and neck cancer, malignant melanoma, and multiple myeloma, highlighting FLAD1′s potential clinical relevance in cancer research and therapy.

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FLAD1 Antibody (G-4) References:

1. The riboflavin/FAD cycle in rat liver mitochondria.  |  Barile, M., et al. 2000. Eur J Biochem. 267: 4888-900. PMID: 10903524
2. Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase.  |  Brizio, C., et al. 2006. Biochem Biophys Res Commun. 344: 1008-16. PMID: 16643857
3. Over-expression in Escherichia coli, purification and characterization of isoform 2 of human FAD synthetase.  |  Galluccio, M., et al. 2007. Protein Expr Purif. 52: 175-81. PMID: 17049878
4. Transient multiple acyl-CoA dehydrogenation deficiency in a newborn female caused by maternal riboflavin deficiency.  |  Chiong, MA., et al. 2007. Mol Genet Metab. 92: 109-14. PMID: 17689999
5. Mitochondrial localization of human FAD synthetase isoform 1.  |  Torchetti, EM., et al. 2010. Mitochondrion. 10: 263-73. PMID: 20060505
6. Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD.  |  Leulliot, N., et al. 2010. J Mol Biol. 398: 641-6. PMID: 20359485
7. Evolutionary divergence of chloroplast FAD synthetase proteins.  |  Yruela, I., et al. 2010. BMC Evol Biol. 10: 311. PMID: 20955574
8. Cloning and characterization of FAD1, the structural gene for flavin adenine dinucleotide synthetase of Saccharomyces cerevisiae.  |  Wu, M., et al. 1995. Mol Cell Biol. 15: 264-71. PMID: 7799934

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FLAD1 Antibody (G-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-FLAD1 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FLAD1 Antibody (G-4) Specifications

Alternate Product Name: FLAD1

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 157-195

Epitope Target: FLAD1

Epitope Species: human

Product Reactivity/Detection: FLAD1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for FLAD1 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin