Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FKBP6 CRISPR/Cas9 KO Plasmid (h): sc-406798
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FKBP6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FKBP6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FKBP6 Antibody (AT9B7): sc-517401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP6 CRISPR/Cas9 KO Plasmid (h) | sc-406798 | 20 µg | $397.00 |
Overview
FKBP6 encodes FK506-binding protein 6, an immunophilin with peptidyl-prolyl cis–trans isomerase activity that supports protein folding and chaperone-dependent maturation in the cytoplasm and nucleus. In the germline, FKBP6 is implicated in meiotic progression and synaptonemal complex function, coordinating protein–protein interactions required for homolog pairing and recombination. Disruption of FKBP6 has been linked to defects in spermatogenesis and male infertility phenotypes, making it a useful entry point for studying reproductive biology and meiosis-associated quality control. Its molecular roles also connect to broader proteostasis networks governed by immunophilins and HSP90-associated complexes that influence stability and trafficking of client proteins.
FKBP6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FKBP6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FKBP6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FKBP6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FKBP6 protein expression.
This CRISPR knockout system enables efficient generation of FKBP6-deficient cell models for investigation of FKBP6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FKBP6 exon(s) critical for FKBP6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FKBP6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FKBP6 CRISPR/Cas9 KO Plasmid (h) and FKBP6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FKBP6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FKBP6 HDR Plasmid (h) and FKBP6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FKBP6 homology arms to support homology-directed repair at defined FKBP6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.