FLG/Filaggrin Antibody (AE21): sc-80609

Filaggrin (AE21) is a mouse monoclonal IgG1 kappa light chain antibody that detects Filaggrin of human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Filaggrin antibody (AE21) is available as non-conjugated form, as well as multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Profilaggrin is a large, insoluble, highly phosphorylated precursor protein containing several tandem copies of a 324 amino acid stretch. Mammalian profilaggrin is a major protein component of keratohyalin granules in living epidermal cells. Keratohyalin granules contribute to the keratin content of dead cornified cells. During terminal differentiation of the epidermis, profilaggrin undergoes proteolytic processing into active filaggrin molecules that promote aggregation and disulfide-bond formation of keratin intermediate filaments. Active filaggrin exists at an epidermal level where keratinocytes transition between the live nucleated granular layer and anucleate cornified layer, suggesting a role in terminal differentiation by facilitating apoptotic machinery.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FLG/Filaggrin Antibody (AE21) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-FLG/Filaggrin Antibody (AE21) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FLG/Filaggrin Antibody (AE21) Specifications

Alternate Product Name: Filaggrin

Product Clone Name: AE21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Filaggrin

Epitope Species: human

Product Reactivity/Detection: Filaggrin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for FLG/Filaggrin Antibody (AE21): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred FLG/Filaggrin antibody is FLG/Filaggrin Antibody (AKH1): sc-66192.