FLG/Filaggrin Antibody (AKH1): sc-66192

Filaggrin Antibody (AKH1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Filaggrin protein of human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Filaggrin antibody (AKH1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Filaggrin, a key structural protein, plays a crucial role in the skin′s barrier function by promoting the aggregation of keratin intermediate filaments, which is essential for maintaining the integrity and resilience of the epidermis. Filaggrin is primarily located in the keratohyalin granules of the epidermis, where synthesis occurs as a large precursor known as profilaggrin. During the terminal differentiation of keratinocytes, profilaggrin undergoes proteolytic cleavage into active filaggrin, which facilitates the formation of a robust cornified envelope that protects against environmental stressors and prevents water loss. Proper functioning of filaggrin is vital for skin health, as mutations in the filaggrin gene are associated with various skin disorders, including atopic dermatitis and ichthyosis vulgaris, highlighting filaggrin′s importance in both skin barrier function and overall dermatological health.

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FLG/Filaggrin Antibody (AKH1) References:

1. Inducible expression of filaggrin increases keratinocyte susceptibility to apoptotic cell death.  |  Kuechle, MK., et al. 2000. Cell Death Differ. 7: 566-73. PMID: 10822280
2. Filaggrin mutations associated with skin and allergic diseases.  |  Irvine, AD., et al. 2011. N Engl J Med. 365: 1315-27. PMID: 21991953
3. Organization, structure, and polymorphisms of the human profilaggrin gene.  |  Gan, SQ., et al. 1990. Biochemistry. 29: 9432-40. PMID: 2248957
4. Filaggrin and Skin Barrier Function.  |  Kezic, S. and Jakasa, I. 2016. Curr Probl Dermatol. 49: 1-7. PMID: 26844893
5. Characterization of a cDNA clone encoding human filaggrin and localization of the gene to chromosome region 1q21.  |  McKinley-Grant, LJ., et al. 1989. Proc Natl Acad Sci U S A. 86: 4848-52. PMID: 2740331
6. The role of filaggrin in atopic dermatitis and allergic disease.  |  Drislane, C. and Irvine, AD. 2020. Ann Allergy Asthma Immunol. 124: 36-43. PMID: 31622670
7. Revisiting the Roles of Filaggrin in Atopic Dermatitis.  |  Moosbrugger-Martinz, V., et al. 2022. Int J Mol Sci. 23: PMID: 35628125
8. PDE4 inhibition by difamilast regulates filaggrin and loricrin expression via keratinocyte proline-rich protein in human keratinocytes.  |  Tsuji, G., et al. 2023. J Dermatol Sci. 110: 61-68. PMID: 37156706
9. Influence of pathogenic filaggrin variants on dupilumab treatment in atopic dermatitis.  |  Clabbers, J., et al. 2024. J Allergy Clin Immunol. 153: 1155-1161.e4. PMID: 38272373
10. Filaggrin Mutation Status and Prevention of Atopic Dermatitis with Maternal Probiotic Supplementation.  |  Zakiudin, DP., et al. 2024. Acta Derm Venereol. 104: adv24360. PMID: 38655655
11. Filaggrin linker segment peptide and cystatin alpha are parts of a complex of the cornified envelope of epidermis.  |  Takahashi, M., et al. 1996. Arch Biochem Biophys. 329: 123-6. PMID: 8619628
12. Recruitment of cycling epidermal cells and expression of filaggrin, involucrin and tenascin in the margin of the active psoriatic plaque, in the uninvolved skin of psoriatic patients and in the normal healthy skin.  |  Gerritsen, MJ., et al. 1997. J Dermatol Sci. 14: 179-88. PMID: 9138475

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FLG/Filaggrin Antibody (AKH1) has 98 citations in a variety of scientific publications.

Primary antibodies like Anti-FLG/Filaggrin Antibody (AKH1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FLG/Filaggrin Antibody (AKH1) Specifications

Alternate Product Name: Filaggrin

Product Clone Name: AKH1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Filaggrin

Epitope Species: human

Product Reactivity/Detection: Filaggrin and Profilaggrin

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 98 citations in various scientific publications

Available Conjugates/Forms for FLG/Filaggrin Antibody (AKH1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred FLG/Filaggrin antibody is FLG/Filaggrin Antibody (AE21): sc-80609.