Anti-Fibulin-1 Antibody (B-5) recommended for detection of Fibulin-1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Fibulin-1 Antibody (B-5): sc-25281

Anti-Fibulin-1 Antibody (B-5) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-Fibulin-1 Antibody (B-5) is a mouse monoclonal IgG2a κ Fibulin-1 antibody, cited in 18 publications, provided at 200 µg/ml
    • Fibulin-1 Antibody (B-5) is recommended for detection of Fibulin-1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Fibulin-1 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fibulin-1 (B-5): sc-25281.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Fibulin-1 Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Fibulin-1 Antibody (B-5) (see ordering information below).
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 14
Date published: 2017-01-26
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Rated 5 out of 5 by from The go to monoclonal for Fibulin-1 Well characterized antibody for IHC(P) and WB in the literature, highly recommended for formalin fixed, paraffin embedded human tissue samples.
Date published: 2016-12-16
Rated 5 out of 5 by from Robust bands observed in western blot analysis Robust bands observed in western blot analysis of Fibulin-1 expression in HeLa and ZR-75-1 whole cell lysates. -SCBT QC
Date published: 2015-05-26
Rated 5 out of 5 by from Exceptional immunoperoxidase membrane staining Exceptional immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human soft tissue. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Published IHC data using human liver Published IHC data using human liver, lymph node, & bone core samples. -SCBT Publication Review
Date published: 2015-03-23
Rated 5 out of 5 by from WB: strong signal at correct molecular weight WB: strong signal at correct molecular weight using CCD-1064Sk whole cell lysate. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Good for detection of fibulin in Western Blot Good for detection of fibulin in Western Blot. Would recommend this one over sc-8674.
Date published: 2015-03-13
Rated 5 out of 5 by from Used for immunoperoxidase staining of paraffin Used for immunoperoxidase staining of paraffin embedded human breast cancer sections. -SCBT Publication Review
Date published: 2015-01-25
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