Fibulin-1 Antibody (B-5): sc-25281
- Fibulin-1 Antibody (B-5) is a mouse monoclonal IgG2a κ Fibulin-1 antibody, cited in 20 publications, provided at 200 µg/ml
- Fibulin-1 Antibody (B-5) is recommended for detection of Fibulin-1 of human origin by WB, IP, IF, IHC(P) and ELISA
- Fibulin-1 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Fibulin-1 Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with Fibulin-1 Antibody (B-5) (see ordering information below).
QUICK LINKS
Fibulin-1 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Fibulin-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Fibulin-1 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fibulin-1 is a crucial modular glycoprotein that plays a significant role in the structure and function of elastic extracellular matrix, basement membranes, and blood vessels. Fibulin-1′s ability to self-associate and bind to key proteins such as calcium, fibronectin, laminin, nidogen, and fibrinogen is vital for maintaining the integrity and elasticity of connective tissues. These interactions are essential for proper formation of basement membranes and elastic fibers, which are critical for tissue resilience and function. Notably, Fibulin-1 expression is influenced by estrogen in ovarian cancer cell lines, suggesting a dual role as a potential agent of metastasis and a biomarker for assessing cancer risk and aggressiveness in ovarian carcinomas. Furthermore, research indicates that increased exposure to Fibulin-1 may inhibit cancer cell motility, highlighting a complex involvement in cancer biology. Anti-Fibulin-1 antibody (B-5) serves as a valuable tool for researchers studying diverse roles in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Fibulin-1 Antibody (B-5) References:
- Complete exon-intron organization of the mouse fibulin-1 gene and its comparison with the human fibulin-1 gene. | Pan, TC., et al. 1999. FEBS Lett. 444: 38-42. PMID: 10037144
- Estrogens increase the expression of fibulin-1, an extracellular matrix protein secreted by human ovarian cancer cells. | Clinton, GM., et al. 1996. Proc Natl Acad Sci U S A. 93: 316-20. PMID: 8552629
- The self-association and fibronectin-binding sites of fibulin-1 map to calcium-binding epidermal growth factor-like domains. | Tran, H., et al. 1997. J Biol Chem. 272: 22600-6. PMID: 9278415
- Estradiol and fibulin-1 inhibit motility of human ovarian- and breast-cancer cells induced by fibronectin. | Hayashido, Y., et al. 1998. Int J Cancer. 75: 654-8. PMID: 9466671
- Estrogen receptor mediated inhibition of cancer cell invasion and motility: an overview. | Rochefort, H., et al. 1998. J Steroid Biochem Mol Biol. 65: 163-8. PMID: 9699869
- Increased immunostaining of fibulin-1, an estrogen-regulated protein in the stroma of human ovarian epithelial tumors. | Roger, P., et al. 1998. Am J Pathol. 153: 1579-88. PMID: 9811350
- Identification of chicken and C. elegans fibulin-1 homologs and characterization of the C. elegans fibulin-1 gene. | Barth, JL., et al. 1998. Matrix Biol. 17: 635-46. PMID: 9923656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibulin-1 Antibody (B-5) | sc-25281 | 200 µg/ml | $316.00 | |||
Fibulin-1 Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-525516 | 200 µg Ab; 10 µg BP | $354.00 | |||
Fibulin-1 Antibody (B-5): m-IgG2a BP-HRP Bundle | sc-546299 | 200 µg Ab; 10 µg BP | $354.00 | |||
Fibulin-1 Antibody (B-5) AC | sc-25281 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Fibulin-1 Antibody (B-5) HRP | sc-25281 HRP | 200 µg/ml | $316.00 | |||
Fibulin-1 Antibody (B-5) FITC | sc-25281 FITC | 200 µg/ml | $330.00 | |||
Fibulin-1 Antibody (B-5) PE | sc-25281 PE | 200 µg/ml | $343.00 | |||
Fibulin-1 Antibody (B-5) Alexa Fluor® 488 | sc-25281 AF488 | 200 µg/ml | $357.00 | |||
Fibulin-1 Antibody (B-5) Alexa Fluor® 546 | sc-25281 AF546 | 200 µg/ml | $357.00 | |||
Fibulin-1 Antibody (B-5) Alexa Fluor® 594 | sc-25281 AF594 | 200 µg/ml | $357.00 | |||
Fibulin-1 Antibody (B-5) Alexa Fluor® 647 | sc-25281 AF647 | 200 µg/ml | $357.00 | |||
Fibulin-1 Antibody (B-5) Alexa Fluor® 680 | sc-25281 AF680 | 200 µg/ml | $357.00 | |||
Fibulin-1 Antibody (B-5) Alexa Fluor® 790 | sc-25281 AF790 | 200 µg/ml | $357.00 |