Fibronectin Antibody (Fn-3): sc-53285

Fibronectin Antibody (Fn-3) is a mouse monoclonal IgG1 antibody that detects Fibronectin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Fibronectin is a crucial extracellular matrix glycoprotein that plays a significant role in various biological processes, including cell adhesion, migration, and wound healing, making Fibronectin essential for tissue repair and regeneration. This high molecular weight heterodimeric protein exists on most cell surfaces, in extracellular fluids, and plasma, highlighting Fibronectin′s widespread importance in maintaining cellular architecture and function. Fibronectin′s structure features multiple domains that facilitate interactions with other proteins and cells, allowing Fibronectin to form a complex network within the extracellular matrix. This structural versatility enables anti-Fibronectin antibody (Fn-3) to bind to integrins and other extracellular matrix components, thereby influencing cellular behavior and tissue organization. Alternative splicing and glycosylation of Fibronectin lead to various isoforms, some associated with specific tissues or pathological conditions, such as malignancies. Notably, glycosylated Fibronectin and splice variants have been linked to myofibroblast phenotype in Dupuytren′s disease, underscoring Fibronectin′s relevance in both normal physiology and disease states. Fibronectin (Fn-3) antibody serves as an invaluable tool for researchers investigating Fibronectin′s multifaceted roles in health and disease.

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Fibronectin Antibody (Fn-3) References:

1. Fibronectin matrix polymerization regulates small airway epithelial cell migration.  |  Hocking, DC. and Chang, CH. 2003. Am J Physiol Lung Cell Mol Physiol. 285: L169-79. PMID: 12639845
2. Assembly of fibronectin into extracellular matrix.  |  Mosher, DF., et al. 1991. Ann N Y Acad Sci. 614: 167-80. PMID: 1673833
3. Emerging roles of fibronectin in thrombosis.  |  Maurer, LM., et al. 2010. Thromb Res. 125: 287-91. PMID: 20116835
4. Cell-Matrix interactions, the role of fibronectin and integrins. A survey.  |  Labat-Robert, J. 2012. Pathol Biol (Paris). 60: 15-9. PMID: 22265966
5. The role of fibronectin in neural development.  |  Rogers, SL., et al. 1989. Dev Neurosci. 11: 248-65. PMID: 2676455
6. Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments.  |  Pietrocola, G., et al. 2017. Methods Mol Biol. 1627: 309-324. PMID: 28836210
7. Characterization of fibronectin properties by integrated micro-fluidic experiments and fluid-structure interaction simulations.  |  Ke, R., et al. 2023. J Biomech. 150: 111505. PMID: 36867952
8. The cell attachment determinant in fibronectin.  |  Pierschbacher, MD., et al. 1985. J Cell Biochem. 28: 115-26. PMID: 3908463
9. Fibronectin: a chromatin-associated protein?  |  Zardi, L., et al. 1979. Cell. 18: 649-57. PMID: 519752
10. Fibronectin matrix assembly enhances adhesion-dependent cell growth.  |  Sottile, J., et al. 1998. J Cell Sci. 111 (Pt 19): 2933-43. PMID: 9730985

The preferred Fibronectin antibody is Fibronectin Antibody (EP5): sc-8422.