Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Fibromodulin CRISPR/Cas9 KO Plasmid (h): sc-401610
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Fibromodulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Fibromodulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Fibromodulin Antibody (H-11): sc-166406
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibromodulin CRISPR/Cas9 KO Plasmid (h) | sc-401610 | 20 µg | $397.00 |
Overview
FMOD encodes fibromodulin, a small leucine-rich proteoglycan of the extracellular matrix that binds collagen fibrils and helps regulate fibrillogenesis, matrix architecture, and tissue mechanical properties. Fibromodulin modulates growth factor signaling, including interactions with TGF-β family pathways, and contributes to cell–matrix communication affecting adhesion, migration, and wound repair programs. Altered FMOD expression or ECM organization has been associated with fibrotic remodeling and dysregulated stromal responses in multiple tissues. As a matrix component influencing collagen deposition and signaling availability, FMOD is frequently studied in connective tissue biology, inflammation-associated remodeling, and tumor microenvironment research.
Fibromodulin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FMOD gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FMOD together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FMOD open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Fibromodulin protein expression.
This CRISPR knockout system enables efficient generation of FMOD-deficient cell models for investigation of Fibromodulin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FMOD exon(s) critical for Fibromodulin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FMOD genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Fibromodulin CRISPR/Cas9 KO Plasmid (h) and Fibromodulin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FMOD locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Fibromodulin HDR Plasmid (h) and Fibromodulin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FMOD homology arms to support homology-directed repair at defined FMOD target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.