fetuin-A CRISPR/Cas9 KO Plasmid (m): sc-419056

Overview

Ahsg encodes fetuin-A, a liver-derived secreted glycoprotein that circulates in plasma and modulates mineral metabolism by binding calcium and phosphate, limiting ectopic calcification through formation of calciprotein particles. Fetuin-A also influences inflammatory signaling and metabolic homeostasis, including effects on insulin receptor–linked pathways and macrophage activation, and is often studied in the context of systemic acute-phase responses. In mouse models, altered fetuin-A levels have been linked to pathological calcification, metabolic dysfunction, and tissue remodeling, making Ahsg a useful node for dissecting crosstalk between hepatic secretion, extracellular matrix mineralization, and innate immune processes. Its extracellular localization and broad binding capacity support investigations into proteomic interactions, serum factor biology, and calcification-prone microenvironments.

fetuin-A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ahsg gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ahsg together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ahsg open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish fetuin-A protein expression.

This CRISPR knockout system enables efficient generation of Ahsg-deficient cell models for investigation of fetuin-A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Ahsg exon(s) critical for fetuin-A function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Ahsg genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by fetuin-A CRISPR/Cas9 KO Plasmid (m) and fetuin-A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ahsg locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by fetuin-A HDR Plasmid (m) and fetuin-A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ahsg homology arms to support homology-directed repair at defined Ahsg target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.