Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ferritin heavy chain CRISPR/Cas9 KO Plasmid (h): sc-418152
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ferritin heavy chain CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ferritin heavy chain genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ferritin heavy chain Antibody (B-12): sc-376594
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ferritin heavy chain CRISPR/Cas9 KO Plasmid (h) | sc-418152 | 20 µg | $397.00 |
Overview
FTH1 encodes the ferritin heavy chain, a catalytic subunit of the ferritin complex that oxidizes Fe2+ to Fe3+ and enables safe intracellular iron storage. By buffering labile iron and limiting iron-driven reactive oxygen species formation, FTH1 contributes to redox homeostasis, mitochondrial function, and protection from oxidative damage. Ferritin dynamics intersect with iron uptake and export pathways, including transferrin receptor–mediated endocytosis and ferroportin-dependent efflux, shaping cellular responses to hypoxia, inflammation, and metabolic stress. Dysregulated FTH1 expression or ferritin metabolism is frequently studied in contexts of iron overload, neurodegeneration, infection-associated inflammation, and tumor biology where iron availability influences proliferation and cell death programs.
ferritin heavy chain CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FTH1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FTH1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FTH1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ferritin heavy chain protein expression.
This CRISPR knockout system enables efficient generation of FTH1-deficient cell models for investigation of ferritin heavy chain signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FTH1 exon(s) critical for ferritin heavy chain function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FTH1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ferritin heavy chain CRISPR/Cas9 KO Plasmid (h) and ferritin heavy chain CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FTH1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ferritin heavy chain HDR Plasmid (h) and ferritin heavy chain HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FTH1 homology arms to support homology-directed repair at defined FTH1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.