ferritin heavy chain Antibody (B-12): sc-376594

ferritin heavy chain Antibody (B-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects ferritin heavy chain (FTH) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-ferritin heavy chain antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FTH plays a crucial role in iron metabolism, primarily by catalyzing the oxidation of ferrous iron (FeII) to ferric iron (FeIII), which is essential for safe iron storage within cells. This process is vital because excess free iron can generate harmful reactive oxygen species, leading to cellular damage. By facilitating the initial step of iron storage, FTH helps maintain cellular iron homeostasis and protects against oxidative stress. Additionally, FTH is implicated in the regulation of thymidine biosynthesis, enhancing the expression of cytoplasmic serine hydroxymethyltransferase, a key enzyme in the synthesis of thymidylate, particularly in MCF-7 cells. Understanding FTH function is important not only for its role in iron storage but also for its involvement in various cellular processes, making FTH a significant target for research in iron-related disorders and cellular health.

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ferritin heavy chain Antibody (B-12) References:

1. High level of ferritin light chain mRNA in lens.  |  Cheng, Q., et al. 2000. Biochem Biophys Res Commun. 270: 349-55. PMID: 10753629
2. Heavy chain ferritin enhances serine hydroxymethyltransferase expression and de novo thymidine biosynthesis.  |  Oppenheim, EW., et al. 2001. J Biol Chem. 276: 19855-61. PMID: 11278996
3. Sulfide is an efficient iron releasing agent for mammalian ferritins.  |  Cassanelli, S. and Moulis, J. 2001. Biochim Biophys Acta. 1547: 174-82. PMID: 11343803
4. Regulation of neuronal ferritin heavy chain, a new player in opiate-induced chemokine dysfunction.  |  Abt, AC. and Meucci, O. 2011. J Neuroimmune Pharmacol. 6: 466-76. PMID: 21465240
5. Counteraction of Myocardial Ferritin Heavy Chain Deficiency by Heme Oxygenase-1.  |  Machado, SE., et al. 2022. Int J Mol Sci. 23: PMID: 35955444
6. Ferritin Heavy Chain Binds Peroxiredoxin 6 and Inhibits Cell Proliferation and Migration.  |  Di Sanzo, M., et al. 2022. Int J Mol Sci. 23: PMID: 36361777
7. M2 Macrophage-Derived Exosomal Ferritin Heavy Chain Promotes Colon Cancer Cell Proliferation.  |  Cui, Z., et al. 2023. Biol Trace Elem Res. 201: 3717-3728. PMID: 36418633
8. Ferritin heavy chain participated in ameliorating 3-nitropropionic acid-induced oxidative stress and apoptosis of goose follicular granulosa cells.  |  Jiang, D., et al. 2023. Poult Sci. 102: 102606. PMID: 36940654
9. Heterozygous nonsense variants in the ferritin heavy-chain gene FTH1 cause a neuroferritinopathy.  |  Shieh, JT., et al. 2023. HGG Adv. 4: 100236. PMID: 37660254
10. Ferritin heavy chain supports stability and function of the regulatory T cell lineage.  |  Wu, Q., et al. 2024. EMBO J. 43: 1445-1483. PMID: 38499786
11. Assignment of human ferritin genes to chromosomes 11 and 19q13.3----19qter.  |  Worwood, M., et al. 1985. Hum Genet. 69: 371-4. PMID: 3857215
12. Comparison of the three-dimensional structures of recombinant human H and horse L ferritins at high resolution.  |  Hempstead, PD., et al. 1997. J Mol Biol. 268: 424-48. PMID: 9159481

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ferritin heavy chain Antibody (B-12) has 79 citations in a variety of scientific publications.

Primary antibodies like Anti-ferritin heavy chain Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ferritin heavy chain Antibody (B-12) Specifications

Alternate Product Name: FTH1

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 131-183

Epitope Target: ferritin heavy chain

Epitope Species: human

Product Reactivity/Detection: ferritin heavy chain

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 79 citations in various scientific publications

Available Conjugates/Forms for ferritin heavy chain Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin