FEN-1 Antibody (B-4): sc-28355

FEN-1 Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects FEN-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FEN-1 Antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FEN-1, also known as flap endonuclease, plays a crucial role in DNA replication and repair, specifically by cleaving the 5′ flap structure of DNA, which is essential for maintaining genome stability. FEN-1 is particularly important because of involvement in processing Okazaki fragments during DNA replication and excising damaged DNA during repair processes. Additionally, FEN-1 is highly homologous to yeast RAD2 protein, and FEN-1′s C-terminal region may interact with proliferating cell nuclear antigen (PCNA), facilitating function as an exonuclease in DNA replication. FEN-1′s ability to ensure accurate DNA replication and repair underscores significance in preventing genomic instability, which can lead to various diseases, including cancer.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FEN-1 Antibody (B-4) References:

1. Biochemical characterization of the WRN-FEN-1 functional interaction.  |  Brosh, RM., et al. 2002. Biochemistry. 41: 12204-16. PMID: 12356323
2. Nuclease-deficient FEN-1 blocks Rad51/BRCA1-mediated repair and causes trinucleotide repeat instability.  |  Spiro, C. and McMurray, CT. 2003. Mol Cell Biol. 23: 6063-74. PMID: 12917330
3. Multiple but dissectible functions of FEN-1 nucleases in nucleic acid processing, genome stability and diseases.  |  Shen, B., et al. 2005. Bioessays. 27: 717-29. PMID: 15954100
4. Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity.  |  Hohl, M., et al. 2007. Nucleic Acids Res. 35: 3053-63. PMID: 17452369
5. Discontinuous DNA synthesis by purified mammalian proteins.  |  Goulian, M., et al. 1990. J Biol Chem. 265: 18461-71. PMID: 2170412
6. RECQ1 interacts with FEN-1 and promotes binding of FEN-1 to telomeric chromatin.  |  Sami, F., et al. 2015. Biochem J. 468: 227-44. PMID: 25774876
7. Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA.  |  Johnson, RE., et al. 1995. Science. 269: 238-40. PMID: 7618086
8. Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human.  |  Hiraoka, LR., et al. 1995. Genomics. 25: 220-5. PMID: 7774922
9. Conditional lethality of null mutations in RTH1 that encodes the yeast counterpart of a mammalian 5'- to 3'-exonuclease required for lagging strand DNA synthesis in reconstituted systems.  |  Sommers, CH., et al. 1995. J Biol Chem. 270: 4193-6. PMID: 7876174
10. Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair.  |  Harrington, JJ. and Lieber, MR. 1994. Genes Dev. 8: 1344-55. PMID: 7926735
11. Reconstitution of complete SV40 DNA replication with purified replication factors.  |  Waga, S., et al. 1994. J Biol Chem. 269: 10923-34. PMID: 8144677
12. Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity.  |  Hosfield, DJ., et al. 1998. Cell. 95: 135-46. PMID: 9778254

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FEN-1 Antibody (B-4) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-FEN-1 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FEN-1 Antibody (B-4) Specifications

Alternate Product Name: FEN-1

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 81-380

Epitope Target: FEN-1

Epitope Species: human

Product Reactivity/Detection: FEN-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for FEN-1 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin