FcRn Antibody (B-8): sc-271745

FcRn Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects FcRn protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). FcRn antibody (B-8) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FcRn protein, also known as FCGRT, plays a crucial role in the immune system by mediating immunoglobulin G (IgG) transport across cellular barriers, particularly in the intestinal epithelium of neonatal mice and rats, where FcRn facilitates selective uptake of IgG from maternal milk, providing passive immunity to newborns. This function is vital for neonatal survival, helping newborns quickly acquire antibodies necessary to combat infections before immune system development. FcRn structure consists of a heavy chain and β-2-microglobulin, with the heavy chain showing approximately 35% amino acid identity to major histocompatibility complex (MHC) class I molecules. This structural similarity enables FcRn to effectively bind and recycle IgG, protecting against lysosomal degradation and ensuring IgG remains the longest-lived plasma protein, essential for maintaining long-term immunity.

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FcRn Antibody (B-8) References:

1. High resolution mapping of the binding site on human IgG1 for Fc gamma RI, Fc gamma RII, Fc gamma RIII, and FcRn and design of IgG1 variants with improved binding to the Fc gamma R.  |  Shields, RL., et al. 2001. J Biol Chem. 276: 6591-604. PMID: 11096108
2. Functional reconstitution of human FcRn in Madin-Darby canine kidney cells requires co-expressed human beta 2-microglobulin.  |  Claypool, SM., et al. 2002. J Biol Chem. 277: 28038-50. PMID: 12023961
3. Membrane-anchored human FcRn can oligomerize in the absence of IgG.  |  Praetor, A., et al. 2002. J Mol Biol. 321: 277-84. PMID: 12144784
4. IgG transcytosis and recycling by FcRn expressed in MDCK cells reveals ligand-induced redistribution.  |  Detmer, SA., et al. 2002. EMBO J. 21: 5953. PMID: 12411512
5. Generation of mutated variants of the human form of the MHC class I-related receptor, FcRn, with increased affinity for mouse immunoglobulin G.  |  Zhou, J., et al. 2003. J Mol Biol. 332: 901-13. PMID: 12972260
6. Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn.  |  Ober, RJ., et al. 2004. J Immunol. 172: 2021-9. PMID: 14764666
7. Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level.  |  Ober, RJ., et al. 2004. Proc Natl Acad Sci U S A. 101: 11076-81. PMID: 15258288
8. Conferring the binding properties of the mouse MHC class I-related receptor, FcRn, onto the human ortholog by sequential rounds of site-directed mutagenesis.  |  Zhou, J., et al. 2005. J Mol Biol. 345: 1071-81. PMID: 15644205
9. Pharmacokinetic effects of 4C9, an anti-FcRn antibody, in rats: implications for the use of FcRn inhibitors for the treatment of humoral autoimmune and alloimmune conditions.  |  Getman, KE. and Balthasar, JP. 2005. J Pharm Sci. 94: 718-29. PMID: 15682382
10. From sorting endosomes to exocytosis: association of Rab4 and Rab11 GTPases with the Fc receptor, FcRn, during recycling.  |  Ward, ES., et al. 2005. Mol Biol Cell. 16: 2028-38. PMID: 15689494
11. Generation and characterization of a high affinity anti-human FcRn antibody, rozanolixizumab, and the effects of different molecular formats on the reduction of plasma IgG concentration.  |  Smith, B., et al. 2018. MAbs. 10: 1111-1130. PMID: 30130439
12. Phase 2 study of efgartigimod, a novel FcRn antagonist, in adult patients with primary immune thrombocytopenia.  |  Newland, AC., et al. 2020. Am J Hematol. 95: 178-187. PMID: 31821591

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FcRn Antibody (B-8) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-FcRn Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FcRn Antibody (B-8) Specifications

Alternate Product Name: neonatal Fc receptor (FCRN)

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 24-297

Epitope Target: FcRn

Epitope Species: human

Product Reactivity/Detection: FcRn

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for FcRn Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin