Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FcRH1 CRISPR/Cas9 KO Plasmid (m): sc-432859
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FcRH1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FcRH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FcRH1 Antibody (E-8): sc-515003
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FcRH1 CRISPR/Cas9 KO Plasmid (m) | sc-432859 | 20 µg | $397.00 |
Overview
Fcrl1 encodes Fc receptor-like 1 (FcRH1), a B cell–associated immunoregulatory receptor implicated in tuning antigen receptor–driven signaling and downstream activation programs. In mouse lymphoid tissues, FcRH1 expression is linked to B cell differentiation states and modulation of proximal signaling events that intersect with pathways controlling calcium flux, MAPK activity, and transcriptional responses following BCR engagement. By influencing thresholds for B cell activation and survival, Fcrl1 provides a functional entry point for studying germinal center dynamics, immune tolerance, and mechanisms that shape antibody responses. Dysregulated Fc receptor–like signaling networks are frequently examined in the context of aberrant B cell phenotypes and immune-associated pathologies, supporting the relevance of Fcrl1 for mechanistic immunology research.
FcRH1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fcrl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fcrl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fcrl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FcRH1 protein expression.
This CRISPR knockout system enables efficient generation of Fcrl1-deficient cell models for investigation of FcRH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Fcrl1 exon(s) critical for FcRH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Fcrl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FcRH1 CRISPR/Cas9 KO Plasmid (m) and FcRH1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fcrl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FcRH1 HDR Plasmid (m) and FcRH1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fcrl1 homology arms to support homology-directed repair at defined Fcrl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.