Fc ε RIγ CRISPR/Cas9 KO Plasmid (m): sc-420303

Overview

Fcer1g encodes the Fc receptor gamma chain (Fc ε RIγ), a signaling adaptor containing an ITAM motif that pairs with multiple immunoreceptors, including FcεRI and Fcγ receptor complexes, to couple ligand engagement to downstream activation. Upon receptor crosslinking, Fc ε RIγ drives SYK-dependent signaling cascades that intersect with PI3K, PLCγ, MAPK, calcium flux, degranulation, cytokine production, and phagocytic programs in myeloid cells and tissue-resident immune populations. In mouse, Fcer1g supports innate immune sensing and effector responses in macrophages, dendritic cells, mast cells, and microglia, shaping inflammatory tone and immune complex handling. Dysregulated Fc ε RIγ-linked pathways are widely studied in allergic inflammation, autoimmune immune-complex pathology, and neuroinflammatory processes where Fc receptor signaling influences tissue damage and remodeling.

Fc ε RIγ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fcer1g gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fcer1g together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fcer1g open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Fc ε RIγ protein expression.

This CRISPR knockout system enables efficient generation of Fcer1g-deficient cell models for investigation of Fc ε RIγ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Fcer1g exon(s) critical for Fc ε RIγ function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Fcer1g genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Fc ε RIγ CRISPR/Cas9 KO Plasmid (m) and Fc ε RIγ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fcer1g locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Fc ε RIγ HDR Plasmid (m) and Fc ε RIγ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fcer1g homology arms to support homology-directed repair at defined Fcer1g target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.