FBXO29 Antibody (D-7): sc-514385
- FBXO29 Antibody (D-7) is a mouse monoclonal IgG1 κ FBXO29 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 284-403 mapping within an internal region of FBXO29 of mouse origin
- FBXO29 Antibody (D-7) is recommended for detection of FBXO29 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FBXO29 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FBXO29 Antibody (D-7) for WB applications. These reagents are now offered in bundles with FBXO29 Antibody (D-7) (see ordering information below).
FBXO29 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBXO29 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBXO29 Antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBXO29, also known as F-box only protein 29 or F-box/WD repeat-containing protein 8 (FBXW8), is a 598 amino acid protein characterized by a 40-amino acid F-box domain and five WD repeats, which are essential for its role in the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex. This complex is crucial for substrate recognition and recruitment, facilitating the ubiquitination and subsequent proteasomal degradation of various target proteins, including cyclins and β-catenin, thereby regulating critical cellular processes such as the cell cycle and immune responses. FBXO29 specifically recognizes and binds to phosphorylated proteins, which is vital for the selective degradation of these substrates, ensuring proper cellular function and homeostasis. Additionally, FBXO29 exists in two isoforms due to alternative splicing, which may contribute to functional diversity in different cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBXO29 Antibody (D-7) References:
- Identification of a family of human F-box proteins. | Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
- A family of mammalian F-box proteins. | Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
- The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. | Craig, KL. and Tyers, M. 1999. Prog Biophys Mol Biol. 72: 299-328. PMID: 10581972
- cDNA cloning and expression analysis of new members of the mammalian F-box protein family. | Ilyin, GP., et al. 2000. Genomics. 67: 40-7. PMID: 10945468
- Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. | Schulman, BA., et al. 2000. Nature. 408: 381-6. PMID: 11099048
- A new subfamily of structurally related human F-box proteins. | Ilyin, GP., et al. 2002. Gene. 296: 11-20. PMID: 12383498
- The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro. | Winston, JT., et al. 1999. Genes Dev. 13: 270-83. PMID: 9990852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBXO29 Antibody (D-7) | sc-514385 | 200 µg/ml | $322.00 | |||
FBXO29 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-531107 | 200 µg Ab; 10 µg BP | $361.00 | |||
FBXO29 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-524705 | 200 µg Ab, 40 µg BP | $361.00 | |||
FBXO29 Antibody (D-7): m-IgG1 BP-HRP Bundle | sc-544400 | 200 µg Ab; 20 µg BP | $361.00 | |||
FBXO29 Antibody (D-7) AC | sc-514385 AC | 500 µg/ml, 25% agarose | $424.00 | |||
FBXO29 Antibody (D-7) HRP | sc-514385 HRP | 200 µg/ml | $322.00 | |||
FBXO29 Antibody (D-7) FITC | sc-514385 FITC | 200 µg/ml | $336.00 | |||
FBXO29 Antibody (D-7) PE | sc-514385 PE | 200 µg/ml | $349.00 | |||
FBXO29 Antibody (D-7) Alexa Fluor® 488 | sc-514385 AF488 | 200 µg/ml | $364.00 | |||
FBXO29 Antibody (D-7) Alexa Fluor® 546 | sc-514385 AF546 | 200 µg/ml | $364.00 | |||
FBXO29 Antibody (D-7) Alexa Fluor® 594 | sc-514385 AF594 | 200 µg/ml | $364.00 | |||
FBXO29 Antibody (D-7) Alexa Fluor® 647 | sc-514385 AF647 | 200 µg/ml | $364.00 | |||
FBXO29 Antibody (D-7) Alexa Fluor® 680 | sc-514385 AF680 | 200 µg/ml | $364.00 | |||
FBXO29 Antibody (D-7) Alexa Fluor® 790 | sc-514385 AF790 | 200 µg/ml | $364.00 |