FBXO25 Antibody (G-5): sc-390219

FBXO25 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBXO25 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBXO25 Antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBXO25, also known as F-box only protein 25, is a 367 amino acid protein characterized by a C-terminal F-box domain, which is essential for its role in the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex. This complex is crucial for substrate recognition and recruitment for ubiquitination, thereby regulating cellular processes such as cell cycle, immune response, signaling pathways, and developmental processes through targeted degradation of proteins like cyclins and b-catenin. FBXO25 is predominantly localized in the nucleus and interacts directly with Skp1 p19 and CUL-1, highlighting its importance in cellular regulation. Disruption of FBXO25 has been linked to X-linked mental retardation, underscoring its significance in neurodevelopmental processes.

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FBXO25 Antibody (G-5) References:

1. Identification of a family of human F-box proteins.  |  Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
2. A family of mammalian F-box proteins.  |  Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
3. Systematic analysis and nomenclature of mammalian F-box proteins.  |  Jin, J., et al. 2004. Genes Dev. 18: 2573-80. PMID: 15520277
4. Characterization of FBX25, encoding a novel brain-expressed F-box protein.  |  Hagens, O., et al. 2006. Biochim Biophys Acta. 1760: 110-8. PMID: 16278047
5. FBXO25, an F-box protein homologue of atrogin-1, is not induced in atrophying muscle.  |  Maragno, AL., et al. 2006. Biochim Biophys Acta. 1760: 966-72. PMID: 16714087
6. FBXO25-associated nuclear domains: a novel subnuclear structure.  |  Manfiolli, AO., et al. 2008. Mol Biol Cell. 19: 1848-61. PMID: 18287534
7. Identification of FBXO25-interacting proteins using an integrated proteomics approach.  |  Teixeira, FR., et al. 2010. Proteomics. 10: 2746-57. PMID: 20473970
8. Molecular characterization of atrogin-1/F-box protein-32 (FBXO32) and F-box protein-25 (FBXO25) in rainbow trout (Oncorhynchus mykiss): Expression across tissues in response to feed deprivation.  |  Cleveland, BM. and Evenhuis, JP. 2010. Comp Biochem Physiol B Biochem Mol Biol. 157: 248-57. PMID: 20601059
9. A novel Fbxo25 acts as an E3 ligase for destructing cardiac specific transcription factors.  |  Jang, JW., et al. 2011. Biochem Biophys Res Commun. 410: 183-8. PMID: 21596019
10. The F-box protein FBXO25 promotes the proteasome-dependent degradation of ELK-1 protein.  |  Teixeira, FR., et al. 2013. J Biol Chem. 288: 28152-62. PMID: 23940030
11. FBXO25 promotes cell proliferation, invasion, and migration of NSCLC.  |  Jiang, GY., et al. 2016. Tumour Biol. 37: 14311-14319. PMID: 27596142
12. ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25.  |  Quintero-Barceinas, RS., et al. 2021. J Biol Chem. 296: 100214. PMID: 33428929

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FBXO25 Antibody (G-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-FBXO25 Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FBXO25 Antibody (G-5) Specifications

Alternate Product Name: FBXO25

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 316-363

Epitope Target: FBXO25

Epitope Species: human

Product Reactivity/Detection: FBXO25

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for FBXO25 Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin