FBP1 Antibody (G-8): sc-271241
- FBP1 Antibody (G-8) is a mouse monoclonal IgG1 κ FBP1 antibody, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 605-641 near the C-terminus of FBP1 of human origin
- FBP1 Antibody (G-8) is recommended for detection of FBP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FBP1 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271241 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FBP1 Antibody (G-8) for WB applications. These reagents are now offered in bundles with FBP1 Antibody (G-8) (see ordering information below).
FBP1 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBP1 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBP1 protein, also known as Far Upstream Element Binding Protein 1, plays a crucial role in regulating the expression of the c-Myc gene, which is essential for cell proliferation and growth. Specifically, FBP1 binds to the far upstream element (FUSE) of the c-Myc gene, facilitating transcription in undifferentiated cells. This interaction is vital because c-Myc is a key regulator of cell cycle progression, apoptosis, and cellular transformation, making FBP1 an important player in cellular growth and differentiation processes. FBP1 expression is significantly reduced upon cellular differentiation, highlighting its role as a growth-dependent regulator. Isolated from proliferating HL-60 cells, FBP1 is part of a family of single-stranded DNA-binding proteins that also includes FBP2 and FBP3, which share a conserved central DNA-binding domain and exhibit significant homology in their carboxyl-terminal activation domains.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBP1 Antibody (G-8) References:
- Structure and dynamics of KH domains from FBP bound to single-stranded DNA. | Braddock, DT., et al. 2002. Nature. 415: 1051-6. PMID: 11875576
- Molecular characterization of MARTA1, a protein interacting with the dendritic targeting element of MAP2 mRNAs. | Rehbein, M., et al. 2002. J Neurochem. 82: 1039-46. PMID: 12358751
- A far upstream element stimulates c-myc expression in undifferentiated leukemia cells. | Avigan, MI., et al. 1990. J Biol Chem. 265: 18538-45. PMID: 2211718
- A transactivator of c-myc is coordinately regulated with the proto-oncogene during cellular growth. | Bazar, L., et al. 1995. Oncogene. 10: 2229-38. PMID: 7784068
- A sequence-specific, single-strand binding protein activates the far upstream element of c-myc and defines a new DNA-binding motif. | Duncan, R., et al. 1994. Genes Dev. 8: 465-80. PMID: 8125259
- Multiple single-stranded cis elements are associated with activated chromatin of the human c-myc gene in vivo. | Michelotti, GA., et al. 1996. Mol Cell Biol. 16: 2656-69. PMID: 8649373
- The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators. | Davis-Smyth, T., et al. 1996. J Biol Chem. 271: 31679-87. PMID: 8940189
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBP1 Antibody (G-8) | sc-271241 | 200 µg/ml | $316.00 | |||
FBP1 Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-529147 | 200 µg Ab; 10 µg BP | $354.00 | |||
FBP1 Antibody (G-8): m-IgGκ BP-HRP Bundle | sc-521889 | 200 µg Ab, 40 µg BP | $354.00 | |||
FBP1 Antibody (G-8): m-IgG1 BP-HRP Bundle | sc-543352 | 200 µg Ab; 20 µg BP | $354.00 | |||
FBP1 Antibody (G-8) X | sc-271241 X | 200 µg/0.1 ml | $316.00 | |||
FBP1 Antibody (G-8) AC | sc-271241 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FBP1 Antibody (G-8) HRP | sc-271241 HRP | 200 µg/ml | $316.00 | |||
FBP1 Antibody (G-8) FITC | sc-271241 FITC | 200 µg/ml | $330.00 | |||
FBP1 Antibody (G-8) PE | sc-271241 PE | 200 µg/ml | $343.00 | |||
FBP1 Antibody (G-8) Alexa Fluor® 488 | sc-271241 AF488 | 200 µg/ml | $357.00 | |||
FBP1 Antibody (G-8) Alexa Fluor® 546 | sc-271241 AF546 | 200 µg/ml | $357.00 | |||
FBP1 Antibody (G-8) Alexa Fluor® 594 | sc-271241 AF594 | 200 µg/ml | $357.00 | |||
FBP1 Antibody (G-8) Alexa Fluor® 647 | sc-271241 AF647 | 200 µg/ml | $357.00 | |||
FBP1 Antibody (G-8) Alexa Fluor® 680 | sc-271241 AF680 | 200 µg/ml | $357.00 | |||
FBP1 Antibody (G-8) Alexa Fluor® 790 | sc-271241 AF790 | 200 µg/ml | $357.00 | |||
FBP1 (G-8) Neutralizing Peptide | sc-271241 P | 100 µg/0.5 ml | $68.00 |