FBL7 Antibody (A-8): sc-374319
- FBL7 Antibody (A-8) is a mouse monoclonal IgG2b κ FBL7 antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 367-405 within an internal region of FBL7 of human origin
- FBL7 Antibody (A-8) is recommended for detection of FBL7 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, porcine and avian
- Anti-FBL7 Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for FBL7 Antibody (A-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with FBL7 Antibody (A-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
FBL7 Antibody (A-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects FBL7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). FBL7 (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBL7 protein, consisting of 491 amino acids and encoded by the human gene FBXL7, plays a crucial role in the ubiquitin-proteasome pathway, primarily through its function as a member of the F-box family. This family of proteins is integral to the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex, which is essential for the recognition and recruitment of substrate proteins for ubiquitination. FBL7 contains a 40 amino acid F-box region and ten leucine-rich repeats, which are vital for interaction with phosphorylated substrates, facilitating ubiquitination and subsequent degradation. FBL7′s ability to target specific proteins, such as cyclins and cyclin-dependent kinase inhibitors, underscores FBL7′s importance in regulating various cellular processes, including the cell cycle, immune responses, and developmental pathways. Localized near the nucleus within the cytoplasm, FBL7 is ubiquitously expressed, highlighting FBL7′s significance in maintaining cellular homeostasis and responding to cellular stress.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBL7 Antibody (A-8) References:
- Identification of a family of human F-box proteins. | Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
- A family of mammalian F-box proteins. | Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
- The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. | Craig, KL. and Tyers, M. 1999. Prog Biophys Mol Biol. 72: 299-328. PMID: 10581972
- cDNA cloning and expression analysis of new members of the mammalian F-box protein family. | Ilyin, GP., et al. 2000. Genomics. 67: 40-7. PMID: 10945468
- Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. | Schulman, BA., et al. 2000. Nature. 408: 381-6. PMID: 11099048
- A new subfamily of structurally related human F-box proteins. | Ilyin, GP., et al. 2002. Gene. 296: 11-20. PMID: 12383498
- The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro. | Winston, JT., et al. 1999. Genes Dev. 13: 270-83. PMID: 9990852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBL7 Antibody (A-8) | sc-374319 | 200 µg/ml | $316.00 | |||
FBL7 Antibody (A-8): m-IgGκ BP-HRP Bundle | sc-522700 | 200 µg Ab, 40 µg BP | $354.00 | |||
FBL7 Antibody (A-8) AC | sc-374319 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FBL7 Antibody (A-8) HRP | sc-374319 HRP | 200 µg/ml | $316.00 | |||
FBL7 Antibody (A-8) FITC | sc-374319 FITC | 200 µg/ml | $330.00 | |||
FBL7 Antibody (A-8) PE | sc-374319 PE | 200 µg/ml | $343.00 | |||
FBL7 Antibody (A-8) Alexa Fluor® 488 | sc-374319 AF488 | 200 µg/ml | $357.00 | |||
FBL7 Antibody (A-8) Alexa Fluor® 546 | sc-374319 AF546 | 200 µg/ml | $357.00 | |||
FBL7 Antibody (A-8) Alexa Fluor® 594 | sc-374319 AF594 | 200 µg/ml | $357.00 | |||
FBL7 Antibody (A-8) Alexa Fluor® 647 | sc-374319 AF647 | 200 µg/ml | $357.00 | |||
FBL7 Antibody (A-8) Alexa Fluor® 680 | sc-374319 AF680 | 200 µg/ml | $357.00 | |||
FBL7 Antibody (A-8) Alexa Fluor® 790 | sc-374319 AF790 | 200 µg/ml | $357.00 | |||
FBL7 (A-8) Neutralizing Peptide | sc-374319 P | 100 µg/0.5 ml | $68.00 |