FBL4 Antibody (A-7): sc-376102
- FBL4 Antibody (A-7) is a mouse monoclonal IgG1 κ FBL4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 551-587 near the C-terminus of FBL4 of human origin
- FBL4 Antibody (A-7) is recommended for detection of FBL4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FBL4 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for FBL4 Antibody (A-7) for WB applications. This reagent is now offered in a bundle with FBL4 Antibody (A-7) (see ordering information below).
FBL4 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBL4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBL4 Antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBL4 protein, consisting of 621 amino acids and encoded by the human gene FBXL4, plays a crucial role in the ubiquitin-proteasome pathway as a member of the F-box family. Notably, FBL4 contains a 40 amino acid F-box region and eight leucine-rich repeats, which are essential for substrate recognition and recruitment within the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex. This complex is vital for regulating various cellular processes, including the cell cycle, immune response, and signaling pathways, by targeting specific proteins such as cyclins and b-catenin for degradation. FBL4 is predominantly localized in the cytoplasm near the nucleus and is expressed in several tissues, including the heart, kidney, liver, lung, pancreas, and placenta, while notably absent in skeletal muscle. FBL4′s ability to modulate protein degradation underscores its importance in maintaining cellular homeostasis and responding to developmental cues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBL4 Antibody (A-7) References:
- Identification of a family of human F-box proteins. | Cenciarelli, C., et al. 1999. Curr Biol. 9: 1177-9. PMID: 10531035
- A family of mammalian F-box proteins. | Winston, JT., et al. 1999. Curr Biol. 9: 1180-2. PMID: 10531037
- The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. | Craig, KL. and Tyers, M. 1999. Prog Biophys Mol Biol. 72: 299-328. PMID: 10581972
- cDNA cloning and expression analysis of new members of the mammalian F-box protein family. | Ilyin, GP., et al. 2000. Genomics. 67: 40-7. PMID: 10945468
- Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. | Schulman, BA., et al. 2000. Nature. 408: 381-6. PMID: 11099048
- A new subfamily of structurally related human F-box proteins. | Ilyin, GP., et al. 2002. Gene. 296: 11-20. PMID: 12383498
- The SCFbeta-TRCP-ubiquitin ligase complex associates specifically with phosphorylated destruction motifs in IkappaBalpha and beta-catenin and stimulates IkappaBalpha ubiquitination in vitro. | Winston, JT., et al. 1999. Genes Dev. 13: 270-83. PMID: 9990852
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBL4 Antibody (A-7) | sc-376102 | 200 µg/ml | $316.00 | |||
FBL4 Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-525998 | 200 µg Ab; 10 µg BP | $354.00 | |||
FBL4 Antibody (A-7) AC | sc-376102 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FBL4 Antibody (A-7) HRP | sc-376102 HRP | 200 µg/ml | $316.00 | |||
FBL4 Antibody (A-7) FITC | sc-376102 FITC | 200 µg/ml | $330.00 | |||
FBL4 Antibody (A-7) PE | sc-376102 PE | 200 µg/ml | $343.00 | |||
FBL4 Antibody (A-7) Alexa Fluor® 488 | sc-376102 AF488 | 200 µg/ml | $357.00 | |||
FBL4 Antibody (A-7) Alexa Fluor® 546 | sc-376102 AF546 | 200 µg/ml | $357.00 | |||
FBL4 Antibody (A-7) Alexa Fluor® 594 | sc-376102 AF594 | 200 µg/ml | $357.00 | |||
FBL4 Antibody (A-7) Alexa Fluor® 647 | sc-376102 AF647 | 200 µg/ml | $357.00 | |||
FBL4 Antibody (A-7) Alexa Fluor® 680 | sc-376102 AF680 | 200 µg/ml | $357.00 | |||
FBL4 Antibody (A-7) Alexa Fluor® 790 | sc-376102 AF790 | 200 µg/ml | $357.00 | |||
FBL4 (A-7) Neutralizing Peptide | sc-376102 P | 100 µg/0.5 ml | $68.00 |