FAST-1/2 Antibody (D-12): sc-377358

FAST-1/2 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects FAST-1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FAST-1/2 (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FAST-1/2, also known as forkhead activin signal transducer-1, plays a crucial role as a transcription factor that interacts with receptor-regulated Smad proteins, particularly Smad2, to bind to activin response elements on DNA. This interaction is vital for regulating gene expression during embryonic development, tumorigenesis, and maintaining differentiated cell states. FAST-1/2′s structure includes a conserved N-terminal forkhead domain essential for DNA binding, which consists of 110 amino acid residues, and a distinct C-terminal Smad interaction domain that facilitates association with various Smad proteins, including Smad2, Smad3, and Smad4. FAST-1 and FAST-2 expression is predominantly observed during early developmental stages, with diminished levels in adult tissues, highlighting their importance in early developmental processes. FAST-1 and FAST-2 DNA binding activity is modulated by transforming growth factor-beta and activin, indicating their role in mediating TGFβ-induced signal transduction pathways.

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FAST-1/2 Antibody (D-12) References:

1. A mouse homologue of FAST-1 transduces TGF beta superfamily signals and is expressed during early embryogenesis.  |  Weisberg, E., et al. 1998. Mech Dev. 79: 17-27. PMID: 10349617
2. The role of FAST-1 and Smads in transcriptional regulation by activin during early Xenopus embryogenesis.  |  Yeo, CY., et al. 1999. J Biol Chem. 274: 26584-90. PMID: 10473623
3. Smad3 inhibits transforming growth factor-beta and activin signaling by competing with Smad4 for FAST-2 binding.  |  Nagarajan, RP., et al. 1999. J Biol Chem. 274: 31229-35. PMID: 10531318
4. Structural basis for the functional difference between Smad2 and Smad3 in FAST-2 (forkhead activin signal transducer-2)-mediated transcription.  |  Nagarajan, RP. and Chen, Y. 2000. Biochem J. 350 Pt 1: 253-9. PMID: 10926851
5. Repeat treatment with icatibant for multiple hereditary angioedema attacks: FAST-2 open-label study.  |  Baş, M., et al. 2013. Allergy. 68: 1452-9. PMID: 24111645
6. Fast 2-D ultrasound strain imaging: the benefits of using a GPU.  |  Idzenga, T., et al. 2014. IEEE Trans Ultrason Ferroelectr Freq Control. 61: 207-13. PMID: 24402909
7. FastViFi: Fast and accurate detection of (Hybrid) Viral DNA and RNA.  |  Javadzadeh, S., et al. 2022. NAR Genom Bioinform. 4: lqac032. PMID: 35493723
8. Development of fast 2.5 MeV neutron detectors for high-intensity stray magnetic field environments.  |  Molin, AD., et al. 2024. Rev Sci Instrum. 95: PMID: 39171980
9. Smad4 and FAST-1 in the assembly of activin-responsive factor.  |  Chen, X., et al. 1997. Nature. 389: 85-9. PMID: 9288972
10. Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein FAST2.  |  Labbé, E., et al. 1998. Mol Cell. 2: 109-20. PMID: 9702197
11. Characterization of human FAST-1, a TGF beta and activin signal transducer.  |  Zhou, S., et al. 1998. Mol Cell. 2: 121-7. PMID: 9702198
12. FAST-2 is a mammalian winged-helix protein which mediates transforming growth factor beta signals.  |  Liu, B., et al. 1999. Mol Cell Biol. 19: 424-30. PMID: 9858566

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FAST-1/2 Antibody (D-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-FAST-1/2 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FAST-1/2 Antibody (D-12) Specifications

Alternate Product Name: FAST-1/2

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 86-365

Epitope Target: FAST-1/2

Epitope Species: human

Product Reactivity/Detection: FAST-1 and FAST-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for FAST-1/2 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent