FANCD2 Antibody (FI17): sc-20022

FANCD2 Antibody (FI17) is a mouse monoclonal IgG1 kappa light chain antibody that detects FANCD2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-FANCD2 antibody (FI17) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FANCD2 plays a crucial role in the DNA damage response, particularly in the repair of interstrand cross-links, which are a type of DNA damage that can lead to genomic instability and cancer if not properly repaired. FANCD2 is part of the Fanconi anemia (FA) pathway, a critical cellular mechanism that maintains genomic integrity. When DNA is damaged, FANCD2 undergoes monoubiquitination, which is essential for activation and subsequent localization to sites of DNA damage, where FANCD2 interacts with other FA proteins and the breast cancer susceptibility protein BRCA1. This interaction is vital for the recruitment of repair factors to the damaged DNA, thereby facilitating effective repair processes. The human FANCD2 gene is located on chromosome 3p25.3, consists of 44 exons, and encodes a 1,451-amino acid nuclear protein that exists in two isoforms, highlighting its complexity and importance in cellular responses to DNA damage.

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FANCD2 Antibody (FI17) References:

1. The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG.  |  de Winter, JP., et al. 2000. Hum Mol Genet. 9: 2665-74. PMID: 11063725
2. Fanconi anemia and DNA repair.  |  Grompe, M. and D'Andrea, A. 2001. Hum Mol Genet. 10: 2253-9. PMID: 11673408
3. A cytoplasmic serine protein kinase binds and may regulate the Fanconi anemia protein FANCA.  |  Yagasaki, H., et al. 2001. Blood. 98: 3650-7. PMID: 11739169
4. Function of the Fanconi anemia pathway in Fanconi anemia complementation group F and D1 cells.  |  Siddique, MA., et al. 2001. Exp Hematol. 29: 1448-55. PMID: 11750104
5. The Chinese hamster FANCG/XRCC9 mutant NM3 fails to express the monoubiquitinated form of the FANCD2 protein, is hypersensitive to a range of DNA damaging agents and exhibits a normal level of spontaneous sister chromatid exchange.  |  Wilson, JB., et al. 2001. Carcinogenesis. 22: 1939-46. PMID: 11751423
6. Phosphorylation of Fanconi anemia protein, FANCA, is regulated by Akt kinase.  |  Otsuki, T., et al. 2002. Biochem Biophys Res Commun. 291: 628-34. PMID: 11855836
7. Structural insight into FANCI-FANCD2 monoubiquitination.  |  Li, L., et al. 2020. Essays Biochem. 64: 807-817. PMID: 32725171
8. FBXL12 degrades FANCD2 to regulate replication recovery and promote cancer cell survival under conditions of replication stress.  |  Brunner, A., et al. 2023. Mol Cell. 83: 3720-3739.e8. PMID: 37591242
9. FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination.  |  Leung, W., et al. 2023. Cell Rep. 42: 113523. PMID: 38060446
10. The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export.  |  Olazabal-Herrero, A., et al. 2024. Cell Rep. 43: 113610. PMID: 38165804
11. FANCD2 deficiency sensitizes SHH medulloblastoma to radiotherapy via ferroptosis.  |  Zhou, H., et al. 2024. J Pathol. 262: 427-440. PMID: 38229567
12. FANCD2-FANCI surveys DNA and recognizes double- to single-stranded junctions.  |  Alcón, P., et al. 2024. Nature. 632: 1165-1173. PMID: 39085614

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FANCD2 Antibody (FI17) has 202 citations in a variety of scientific publications.

Primary antibodies like Anti-FANCD2 Antibody (FI17) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FANCD2 Antibody (FI17) Specifications

Alternate Product Name: FA complementation group D2 (FANCD2)

Product Clone Name: FI17

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-272

Epitope Target: FANCD2

Epitope Species: human

Product Reactivity/Detection: S and L isoforms of FANCD2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 202 citations in various scientific publications

Available Conjugates/Forms for FANCD2 Antibody (FI17): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin