Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FAM118B CRISPR/Cas9 KO Plasmid (h): sc-413182
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM118B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM118B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAM118B Antibody (C-11): sc-514039
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM118B CRISPR/Cas9 KO Plasmid (h) | sc-413182 | 20 µg | $397.00 |
Overview
FAM118B encodes a poorly characterized human protein that is predicted to participate in fundamental cellular homeostasis, with emerging evidence linking FAM118 family members to RNA-associated processes and nuclear organization. Basal expression of FAM118B across tissues suggests a role in maintaining core gene expression programs that intersect with cell-cycle progression, stress responses, and proteostasis networks. Altered expression patterns have been reported in multi-omic cancer datasets and other disease contexts, supporting its use as a candidate regulator in studies of tumor biology and cell fitness. Because its molecular function remains incompletely defined, FAM118B is well suited for hypothesis-driven interrogation using genetic perturbation to map downstream pathways and interaction partners.
FAM118B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FAM118B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FAM118B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FAM118B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM118B protein expression.
This CRISPR knockout system enables efficient generation of FAM118B-deficient cell models for investigation of FAM118B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FAM118B exon(s) critical for FAM118B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FAM118B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM118B CRISPR/Cas9 KO Plasmid (h) and FAM118B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FAM118B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM118B HDR Plasmid (h) and FAM118B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FAM118B homology arms to support homology-directed repair at defined FAM118B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.