Factor V Antibody (6A5): sc-13512
- Factor V Antibody (6A5) is a mouse monoclonal IgG1 κ Factor V antibody provided at 200 µg/ml
- raised against the central portion of Factor V of Factor V light chain of human origin
- Factor V Antibody (6A5) is recommended for detection of Factor V of human origin by WB and IP
- Anti-Factor V Antibody (6A5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Factor V Antibody (6A5) for WB applications. These reagents are now offered in bundles with Factor V Antibody (6A5) (see ordering information below).
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Factor V Antibody (6A5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Factor V protein of human origin by western blotting (WB) and immunoprecipitation (IP). Factor V (6A5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Factor V protein, a 2,196 amino acid single-chain glycoprotein, plays a crucial role in the blood coagulation cascade, which is vital for hemostasis following tissue injury. Upon cleavage by thrombin, Factor V is converted into an active, calcium-dependent dimer known as Factor Va. This heterodimer is essential for the formation of the prothrombinase complex, which, in conjunction with catalytic Factor Xa and calcium ions on the surface of platelets or endothelial cells, facilitates the conversion of prothrombin to active thrombin. Factor V not only promotes coagulation but also has anticoagulant properties when acting as a cofactor for activated protein C, which selectively degrades Factor Va and Factor Xa. Consequently, mutations or alterations in the Factor V gene can lead to significant clinical conditions, such as hemorrhagic diathesis or thrombosis, underscoring the importance of Factor V in maintaining hemostatic balance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Factor V Antibody (6A5) References:
- Crystal structures of the membrane-binding C2 domain of human coagulation factor V. | Macedo-Ribeiro, S., et al. 1999. Nature. 402: 434-9. PMID: 10586886
- The coagulation cascade: initiation, maintenance, and regulation. | Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
- Basic mechanisms in blood coagulation. | Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
- Cloning of a cDNA coding for human factor V, a blood coagulation factor homologous to factor VIII and ceruloplasmin. | Kane, WH. and Davie, EW. 1986. Proc Natl Acad Sci U S A. 83: 6800-4. PMID: 3092220
- Complete cDNA and derived amino acid sequence of human factor V. | Jenny, RJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 4846-50. PMID: 3110773
- Platelet coagulation factor Va: the major secretory platelet phosphoprotein. | Rand, MD., et al. 1994. Blood. 83: 2180-90. PMID: 8161784
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Factor V Antibody (6A5) | sc-13512 | 200 µg/ml | $322.00 | |||
Factor V Antibody (6A5): m-IgG Fc BP-HRP Bundle | sc-526548 | 200 µg Ab; 10 µg BP | $361.00 | |||
Factor V Antibody (6A5): m-IgG1 BP-HRP Bundle | sc-531921 | 200 µg Ab; 20 µg BP | $361.00 | |||
Factor V Antibody (6A5) AC | sc-13512 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Factor V Antibody (6A5) HRP | sc-13512 HRP | 200 µg/ml | $322.00 | |||
Factor V Antibody (6A5) FITC | sc-13512 FITC | 200 µg/ml | $336.00 | |||
Factor V Antibody (6A5) PE | sc-13512 PE | 200 µg/ml | $349.00 | |||
Factor V Antibody (6A5) Alexa Fluor® 488 | sc-13512 AF488 | 200 µg/ml | $364.00 | |||
Factor V Antibody (6A5) Alexa Fluor® 546 | sc-13512 AF546 | 200 µg/ml | $364.00 | |||
Factor V Antibody (6A5) Alexa Fluor® 594 | sc-13512 AF594 | 200 µg/ml | $364.00 | |||
Factor V Antibody (6A5) Alexa Fluor® 647 | sc-13512 AF647 | 200 µg/ml | $364.00 | |||
Factor V Antibody (6A5) Alexa Fluor® 680 | sc-13512 AF680 | 200 µg/ml | $364.00 | |||
Factor V Antibody (6A5) Alexa Fluor® 790 | sc-13512 AF790 | 200 µg/ml | $364.00 |