Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
F4/80 CRISPR/Cas9 KO Plasmid (m): sc-420168
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- F4/80 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the F4/80 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: F4/80 Antibody (C-7): sc-377009
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
F4/80 CRISPR/Cas9 KO Plasmid (m) | sc-420168 | 20 µg | $397.00 |
Overview
Adgre1 encodes the mouse F4/80 antigen (EMR1), an EGF-TM7 family adhesion G protein–coupled receptor prominently expressed on tissue-resident macrophages and related myeloid populations. F4/80 is commonly used to identify macrophage subsets and has been implicated in regulating macrophage differentiation, tissue localization, and immune homeostasis through cell–cell interactions and environmental sensing. Its expression patterns intersect with pathways controlling innate immune activation, antigen presentation, and inflammatory cytokine networks within tissue microenvironments. Altered macrophage abundance or polarization associated with Adgre1/F4/80 expression is frequently studied in inflammatory disorders, metabolic disease, infection biology, neuroinflammation, and tumor-associated macrophage biology.
F4/80 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Adgre1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Adgre1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Adgre1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish F4/80 protein expression.
This CRISPR knockout system enables efficient generation of Adgre1-deficient cell models for investigation of F4/80 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Adgre1 exon(s) critical for F4/80 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Adgre1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by F4/80 CRISPR/Cas9 KO Plasmid (m) and F4/80 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Adgre1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by F4/80 HDR Plasmid (m) and F4/80 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Adgre1 homology arms to support homology-directed repair at defined Adgre1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.