F4/80 Antibody (C-7): sc-377009

F4/80 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects F4/80 protein of mouse, rat, and human origin by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). F4/80 (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. F4/80 protein, also known as EMR1, is a member of the epidermal growth factor (EGF)-TM7 family of G-protein coupled receptors, characterized by extended extracellular regions containing multiple EGF-like domains. F4/80 protein is exclusively expressed on macrophage surfaces, serving as a critical marker for mature macrophage populations in various tissues, including Kupffer cells in the liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 expression on macrophages is vital for understanding immune responses, playing a role in identifying and characterizing these immune cells in both health and disease. F4/80/EMR1 undergoes significant post-translational modifications, including extensive N-linked glycosylation and some O-linked glycosylation, which may influence macrophage adhesion, migration, and signaling pathways. F4/80 expression remains crucial for studying macrophage-related processes in various physiological and pathological contexts, though its precise role in macrophage biology continues to be actively researched.

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F4/80 Antibody (C-7) References:

1. Human EMR2, a novel EGF-TM7 molecule on chromosome 19p13.1, is closely related to CD97.  |  Lin, HH., et al. 2000. Genomics. 67: 188-200. PMID: 10903844
2. The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells.  |  Kwakkenbos, MJ., et al. 2002. J Leukoc Biol. 71: 854-62. PMID: 11994511
3. Inactivation of the F4/80 glycoprotein in the mouse germ line.  |  Schaller, E., et al. 2002. Mol Cell Biol. 22: 8035-43. PMID: 12391169
4. Functional analysis of the murine Emr1 promoter identifies a novel purine-rich regulatory motif required for high-level gene expression in macrophages.  |  O'Reilly, D., et al. 2004. Genomics. 84: 1030-40. PMID: 15533720
5. EMR1, an unusual member in the family of hormone receptors with seven transmembrane segments.  |  Baud, V., et al. 1995. Genomics. 26: 334-44. PMID: 7601460
6. The macrophage cell surface glycoprotein F4/80 is a highly glycosylated proteoglycan.  |  Haidl, ID. and Jefferies, WA. 1996. Eur J Immunol. 26: 1139-46. PMID: 8647179
7. Development of microglia and macrophages in the postnatal rat pituitary.  |  Mander, TH. and Morris, JF. 1996. Cell Tissue Res. 286: 347-55. PMID: 8929337
8. Chromosome mapping of the Emr1 gene.  |  McKnight, AJ., et al. 1997. Mamm Genome. 8: 946. PMID: 9383301
9. Physical mapping of EMR1 and CD97 in human Chromosome 19 and assignment of Cd97 to mouse Chromosome 8 suggest an ancient genomic duplication.  |  Carver, EA., et al. 1999. Mamm Genome. 10: 1039-40. PMID: 10501980

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-F4/80 Antibody (C-7) has 173 citations in a variety of scientific publications.

Primary antibodies like Anti-F4/80 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

F4/80 Antibody (C-7) Specifications

Alternate Product Name: F4/80

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 335-634

Epitope Target: F4/80

Epitope Species: mouse

Product Reactivity/Detection: EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 173 citations in various scientific publications

Available Conjugates/Forms for F4/80 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin