F-Spondin CRISPR/Cas9 KO Plasmid (h): sc-404101
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- F-Spondin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the F-Spondin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: F-Spondin Antibody (B-3): sc-390182
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
F-Spondin CRISPR/Cas9 KO Plasmid (h) | sc-404101 | 20 µg | $397.00 |
Overview
SPON1 encodes F-spondin, an extracellular matrix–associated, secreted glycoprotein enriched in the nervous system that modulates cell–matrix interactions, neurite outgrowth, and axon guidance during development and tissue remodeling. F-spondin can influence adhesion and migration by engaging integrin and proteoglycan-dependent signaling and by shaping extracellular matrix organization. It has been linked to regulation of synaptic connectivity and neuronal plasticity, and altered SPON1 expression has been reported in contexts of neurodegeneration and injury-associated remodeling. As an ECM cue, F-spondin is also studied for its roles in vascular and fibrotic processes where matrix signaling and cell motility are central.
F-Spondin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SPON1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SPON1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SPON1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish F-Spondin protein expression.
This CRISPR knockout system enables efficient generation of SPON1-deficient cell models for investigation of F-Spondin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SPON1 exon(s) critical for F-Spondin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SPON1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by F-Spondin CRISPR/Cas9 KO Plasmid (h) and F-Spondin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SPON1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by F-Spondin HDR Plasmid (h) and F-Spondin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SPON1 homology arms to support homology-directed repair at defined SPON1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.